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Rat hypoxia-inducible factor 1α,HIF-1α ELISA Kit使用原理
大鼠低氧诱导因子1α(HIF-1α)ELISA试剂盒
规格：96T/48T
The kit was a solidphase sandwich enzyme-linked immunosorbent assay (ELISA), and the AVPconcentrations of the standard samples and unknown concentrations ofsamples were detected by the addition of microporous enzyme plates.. Atthe same time, the AVP and the biotin labeled antibodies were incubatedat the same time.. After washing, the addition of Pro labeled HRP. Afterthe warming and washing, the removal of the non binding enzymeconjugate, and then added to the substrate a, B, and the enzymeconjugate at the same time. Produce color. The color depth and theconcentration of AVP in the sample was proportional to the concentrationof the sample..
Operation notes
2 the experimental plate should be immediately put back in the bag,seal the preservation, lest metamorphism.
4 use a disposable suction head lest cross contamination, draw thetermination liquid and substrate B, a liquid, to avoid using the metalpart of the sample.
6 wash the enzyme plate should be fully dry, do not absorb water paperdirectly into the enzyme labeled pores in the water absorption.
avoiding prolonged exposure to light.To avoid contact with hand, toxic. After the experiment, read the ODvalue immediately.
9 to carry out the temperature fertility operation according to thetime, the amount and the order of the liquid added in the instruction manual.
Kit performance:
The 2 batch and the batch should be less than 9% and 15% respectively.
Detection range:
Save condition and its validity:
2 validity: 6 months
