美国ABI NativePAGE™ Novex® 4- BiProtein Gels

 The NativePAGE™ Novex® Bis-Tris Gel System is a pre-cast polyacrylamide mini gel system that provides a sensitive ａnd high-resolution method for analysis of native membrane protein complexes, native soluble proteins, molecular mass estimations, ａnd an assessment of the purity of native proteins. It is based on the blue native polyacrylamide gel electrophoresis (BN PAGE) technique developed by Schagger ａnd von Jagow (1–3). With the NativePAGE™ Novex® Bis-Tris Gel System, you will:

• Simplify resolution of large proteins (15–10,000 kDa)
• Analyze membrane-protein complexes in their native conformations
• Obtain better resolution than with traditional Tris-glycine native electrophoresis

In SDS-PAGE, SDS functions as a charge-shift molecule that denatures proteins by conferring on them a net negative charge ａnd enables proteins to migrate towards the anode. BN PAGE uses Coomassie® G-250 as the charge-shift molecule, which binds to proteins ａnd confers a net negative charge while maintaining the proteins in their nondenatured, native state.

The near neutral pH of the NativePAGE™ Novex® Bis-Tris Gel System provides maximum stability of both the proteins ａnd the gel matrix, resulting in a highly sensitive method for analysis of native membrane-protein complexes ａnd offering superior band resolution over traditional Tris-glycine gel systems.

References
1. Schagger, H. ａnd von Jagow, G. (1991) Anal. Biochem. 199: 223-231.
2. Schagger, H., Cramer, W.A. ａnd von Jagow, G. (1994) Anal. Biochem. 217: 220-230.
3. Schagger, H. (2001) Meth. Cell Biol. 65: 231-244.
规格

Gel Percentage:	4-16 %
Number of Wells:	10 wells
Gel Type:	Native Gel, Bis-Tris Gel
Separation Range:	15 kDa to 1,000 kDa
Gel Size:	Mini (8 cm x 8 cm)
Well Format:	1D Well
Thickness:	1.0 mm
Product Size:	1 box
Mode of Separation:	Molecular Weight

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