美国Rubicon Genomics－－-单细胞测序文库构建的领导品Pai
1. 二代测序建库试剂（微量起始样品 50pg - 50ng）
2. 单细胞扩增和单细胞测序建库试剂（广泛应用于生殖领域，是该领域的金标准。）
3. 全RNA扩增试剂 （微量起始样品，可以低至10个细胞的总RNA）
Rubicon Genomics成立于2000年，总部位于美国密歇根州。该公司的核心竞争力产品包括酶学、核酸化学、样品的加工生产，该公司致力于创新、知识产权的发展和持续的产品开发。
Rubicon Genomics开发创新、高品质的、核酸文库的制备及配套产品，血浆游离DNA基因测序试剂盒能够对临床样本进行简单、快速可靠和高灵敏度的分析。
Rubicon Genomics，总部位于美国密歇根州，产品主要包括以下几类：
【血浆游离DNA基因测序试剂盒主要产品】
1. ThruPLEX® Plasma-seq Kit
2. ThruPLEX® DNA-seq Kit
3. PicoPLEX® DNA-seq Kit
4. PicoPLEX® WGA Kit
5. TransPLEX® C-WTA Kit
【血浆游离DNA基因测序试剂盒产品应用】
1. 外显子组富集
2. SureSelect® 富集
3. SeqCap® EZ 富集
4. 固定石蜡包埋DNA测序
5. 胚胎植入前遗传学筛查(PGS）/种植前遗传学诊断（PGD）
6. DNA测序
7. RNA测序
8. 染色体免疫共沉淀测序
【产品详情】
| 产品名称 | 平台 | 应用程序 | 样本 | 样本量 | 技术 |
| ThruPLEX®DNA-seq Kit | Illumina NGS | WGS, WES, 靶向测序， 染色体免疫共沉淀测序, 转录组测序 | FFPE DNA, gDNA, cDNA, | 50 pg – 50 ng | 高灵敏度基于连接的扩增 |
| ThruPLEX®-FD Prep Kit | Illumina NGS | WGS, WES, 靶向测序，ctDNA分析 | Cell-free DNA isolated from plasma | <1 ng – 30 ng | 高灵敏度基于连接的扩增 |
| PicoPLEX™ DNA-seq Kit | Illumina NGS | CNV,Aneuploidy | Eukaryotic Cells | <6 pg – 60pg | 准随机引物线性扩增 |
| PicoPLEX™ WGA Kit | 阵列，PCR | CNV,Aneuploidy | Eukaryotic Cells | <6 pg – 60 pg DNA | 准随机引物线性扩增 |
| TransPLEX®C-WTA Kit | 阵列，PCR | 基因表达分析 | RNA | 10 – 300 ng RNA | 准随机引物扩增 |
欢迎您致电 华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司 手机号：152 1681 4001。华雅再生医学-客服QQ: 316 808 6348
提供产品：华雅再生医学旗舰公司-红荣微再（上海）生物工程技术有限公司为ZG再生医学和生物工程技术领域的组织器官再生研究、组织器官功能重建研究、干细胞技术研究、组织工程生物支架材料研究、器官移植YL器械产品等再生医学产业链的相关客户提供：iPS/ES胚胎干细胞的种子细胞、心肌细胞、人源肝脏细胞、MSC间充质干细胞、基底膜基质胶细胞支架、干细胞生长分化调控因子、干细胞药物研究的小分子YZ剂、调节剂、小分子化合物、功能胶原蛋白生物材料、3D细胞培养基质胶、器官模型3D打印技术材料、神经干细胞培养基、STEM121抗体、BD科研P100、P800蛋白质组学采血管、BD血浆准备管PPT管、BD PAXgene全血RNA样本收集管、BD公司CPT/PRP美容采血管、Streck Cell-Free RNA BCT游离RNA样本收集管、Streck Cell-Free DNA Urine Preserve游离DNA尿液保存管、Streck Cell-Free DNA BCT游离DNA采血管、循环肿瘤细胞CTC及无创产前基因检测采血管、illumina高品质NGS文库制备试剂盒、ThruPLEX ctDNA测序试剂盒、全基因组测序、全外显子测序、靶向测序、ChIP-Seq测序、CNV和染色体异倍性分析、Rubicon Genomics单细胞全基因组测序试剂盒、生物医学试剂耗材仪器等种类丰富的产品，以“Focus on Tissue ａnd Organ Regeneration专注组织器官再生与功能重建”为己任，我们与客户携手为ZG再生医学产业的发展而前行。
Overview
ThruPLEX® Plasma-seq is powered by ThruPLEX chemistry to generate high performance NGS libraries from cell-free DNA isolated from plasma.
The chemistry is optimized specifically for cell-free DNA to maximize the library complexity ａnd to preserve the GC representation of the input DNA. Libraries can be used for CNV analysis, whole genome sequencing applications or enriched using your custom panels or leading target enrichment platforms, such as Agilent SureSelect® ａnd Roche NimbleGen SeqCap® EZ.
Libraries prepared with ThruPLEX Plasma-seq provide added sensitivity to better understand genetic variations found within the plasma samples. Plasma, along with other liquid biopsies, has gained significant interest in the research community as a valuable ａnd easily available sample type. These communities include translational research in oncology ａnd developmental research involving the fetal fraction derived from maternal plasma.
ThruPLEX® Plasma-seq Kit is intended for Research Use Only. It may not be used for any other purposes including, but not limited to, use in diagnostics, forensics, therapeutics, or in humans. ThruPLEX Plasma-seq may not be transferred to third parties, resold, modified for resale or used to manufacture commercial products without prior written approval of Rubicon Genomics, Inc.ThruPLEX Plasma-seq Kit is protected by U.S. Patents 7,803,550; 8,071,312; 8,399,199; 8,728,737 ａnd corresponding foreign patents. Additional patents are pending.
Designed for cell-free DNA: newly formulated repair ａnd ligation reagents with optimized protocols
High performance NGS libraries: high diversity with broad ａnd reproducible GC coverage
Variable sample input: <1 ng to 30 ng of cell-free DNA
Fast ａnd simple workflow: 3 steps in a single tube or well in 2 hours with no purification or sample transfer steps
Research applications: libraries from samples where results require high sensitivity including liquid biopsies, ctDNA, ａnd targeted sequencing
Automation-friendly: Beckman® FXP Workstation
Highest Diversity, Fewest Unmapped Reads from Cell-Free DNA
ThruPLEX Plasma-seq Kit generated quality libraries with high diversity ａnd a low number of duplicates ａnd unmapped reads. Cell-free DNA was extracted from 3 plasma samples, ａnd libraries were prepared at the amounts indicated as measured by Qubit庐. The amount of mononucleosomal DNA in each sample as measured by the Bioanalyzer庐 corresponded to 0.09 ng, 0.62 ng, ａnd 15.44 ng. Pooled libraries were sequenced on an Illumina NextSeq庐 500 as a paired-end run with 17M to 25M reads per library, Duplication rates were calculated after down-sampling the data to 17 M reads per library.
欢迎您致电 华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司 手机号：152 1681 4001。华雅再生医学-客服QQ: 316 808 6348
提供产品：华雅再生医学旗舰公司-红荣微再（上海）生物工程技术有限公司为ZG再生医学和生物工程技术领域的组织器官再生研究、组织器官功能重建研究、干细胞技术研究、组织工程生物支架材料研究、器官移植YL器械产品等再生医学产业链的相关客户提供：iPS/ES胚胎干细胞的种子细胞、心肌细胞、人源肝脏细胞、MSC间充质干细胞、基底膜基质胶细胞支架、干细胞生长分化调控因子、干细胞药物研究的小分子YZ剂、调节剂、小分子化合物、功能胶原蛋白生物材料、3D细胞培养基质胶、器官模型3D打印技术材料、神经干细胞培养基、STEM121抗体、BD科研P100、P800蛋白质组学采血管、BD血浆准备管PPT管、BD PAXgene全血RNA样本收集管、BD公司CPT/PRP美容采血管、Streck Cell-Free RNA BCT游离RNA样本收集管、Streck Cell-Free DNA Urine Preserve游离DNA尿液保存管、Streck Cell-Free DNA BCT游离DNA采血管、循环肿瘤细胞CTC及无创产前基因检测采血管、illumina高品质NGS文库制备试剂盒、ThruPLEX ctDNA测序试剂盒、全基因组测序、全外显子测序、靶向测序、ChIP-Seq测序、CNV和染色体异倍性分析、Rubicon Genomics单细胞全基因组测序试剂盒、生物医学试剂耗材仪器等种类丰富的产品，以“Focus on Tissue ａnd Organ Regeneration专注组织器官再生与功能重建”为己任，我们与客户携手为ZG再生医学产业的发展而前行。
Outstanding Target Enrichment Peformance
ThruPLEX Plasma-seq Kit libraries were captured at high efficiency ａnd generated data with deep coverage of the kinome for mutation detection. Libraries were prepared from 3 plasma samples at input amounts of 5 ng, 6.5 ng, ａnd 10 ng in triplicate, ａnd targeted sequencing was carried out on an Illumina MiSeq using samples enriched with the ClearSeq Human DNA Kinome Panel for SureSelectXT2. On average, 5M reads were generated per library. Selected bases were successfully captured bases that were in or within 250 bp of the baits.
ThruPLEX Plasma-seq Kit provided the most reproducible ａnd unbiased GC coverage across the human genome. ThruPLEX libraries showed minimal variability across 9 individual plasma samples tested. Libraries were prepared from cell-free DNA isolated from 1 mL of plasma samples ａnd sequenced on an Illumina NextSeq 500. Four separate plasma samples were used to construct the NEBNext Ultra libraries.
Illumina NGS libraries were prepared with 5 ng of cell-free DNA isolated from pooled plasma samples using ThruPLEX Plasma-seq Kit. Low-pass WGS was conducted on a HiSeq with approximately 12.5M reads per library. (Data courtesy of the Karolinska Institute, Sweden).
Starting with 1 to 30 ng of cell-free DNA, ThruPLEX Plasma-seq Kit creates indexed libraries in 3 simple steps: end repair, adapter ligation, ａnd high-fidelity library amplification. No purification or sample transfer steps are required. The streamlined workflow is performed in 2 hours in a single tube or well, preventing sample loss ａnd enhancing positive sample identification.
ThruPLEX Plasma-seq Kit technology is a 3-step reaction that is optimized for cell-free DNA. Cell-free DNA (1 ng to 30 ng) is first repaired in a highly efficient process. Background is reduced using double-stranded adapters with no single-stranded tails. Blunt-end ligation occurs with high-efficiency. Blocked 5’ ends reduce adapter-adapter ligation. Background is further reduced by destroying unused adapters after ligation. A high-fidelity amplification completes the reaction to generate indexed Illumina libraries.
欢迎您致电 华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司 手机号：152 1681 4001。华雅再生医学-客服QQ: 316 808 6348
提供产品：华雅再生医学旗舰公司-红荣微再（上海）生物工程技术有限公司为ZG再生医学和生物工程技术领域的组织器官再生研究、组织器官功能重建研究、干细胞技术研究、组织工程生物支架材料研究、器官移植YL器械产品等再生医学产业链的相关客户提供：iPS/ES胚胎干细胞的种子细胞、心肌细胞、人源肝脏细胞、MSC间充质干细胞、基底膜基质胶细胞支架、干细胞生长分化调控因子、干细胞药物研究的小分子YZ剂、调节剂、小分子化合物、功能胶原蛋白生物材料、3D细胞培养基质胶、器官模型3D打印技术材料、神经干细胞培养基、STEM121抗体、BD科研P100、P800蛋白质组学采血管、BD血浆准备管PPT管、BD PAXgene全血RNA样本收集管、BD公司CPT/PRP美容采血管、Streck Cell-Free RNA BCT游离RNA样本收集管、Streck Cell-Free DNA Urine Preserve游离DNA尿液保存管、Streck Cell-Free DNA BCT游离DNA采血管、循环肿瘤细胞CTC及无创产前基因检测采血管、illumina高品质NGS文库制备试剂盒、ThruPLEX ctDNA测序试剂盒、全基因组测序、全外显子测序、靶向测序、ChIP-Seq测序、CNV和染色体异倍性分析、Rubicon Genomics单细胞全基因组测序试剂盒、生物医学试剂耗材仪器等种类丰富的产品，以“Focus on Tissue ａnd Organ Regeneration专注组织器官再生与功能重建”为己任，我们与客户携手为ZG再生医学产业的发展而前行。
2017年1月，Rubicon Genomics，Inc.成为Takara Bio的一员，现在宝日医生物技术（北京）有限公司（Takara Bio北京分公司）开始在国内销售Rubicon公司开发的下一代测序试剂。Rubicon公司成立于2000年，公司总部位于美国密歇根州。Rubicon公司开发的产品主要基于“ThruPLEX®技术”和“PicoPLEX®技术”，可以微量DNA起始构建下一代测序文库。ThruPLEX®技术”可从血浆中的微量游离DNA构建高多样性文库，准确检测低丰度DNA变异，用途广泛。“PicoPLEX®技术”，可从单细胞等微量DNA起始构建高重复再现性文库，用于拷贝数变异分析和染色体异倍性分析。Rubicon产品丰富了Takara基于SMART®专利技术的下一代测序产品线，为科研工作者在基因组学、单细胞和临床应用研究等领域提供更多解决方案。代表产品之一为：Rubicon Genomics RB3050/R30050 PicoPLEX® WGA Kit 单细胞全基因组扩增试剂盒Whole Genome Amplification from Single Cells。
2016年年底，TAKARA宝日医生物技术（北京）有限公司的市场部部长与宝生物工程（大连）有限公司的销售部部长分别从北京和大连飞往上海，对华雅再生医学的旗舰营销公司：红荣微再（上海）生物工程技术有限公司进行了实地考察，双方深入地探讨了未来干细胞及再生医学市场发展的趋势和方向，在双方公司中长期发展战略上，志同道合地看好再生医学市场的发展前景，历史性的YL革命是我们这代人肩负的伟大使命！
TAKARA公司日本总部是：“iPS干细胞之父”诺贝尔奖获得者的故乡，具有Z前沿的iPS干细胞技术实力，TAKARA公司旗下的Cellartis子公司，拥有超过40人的研发团队，拥有超过15年的人干细胞研究经验，建立并储存大量的人iPS和ES细胞系。TAKARA旗下的Stem Cells公司SC Proven系列：凭借20多年干细胞技术经验研发，包括干细胞研究用培养基、抗体等耗材，具有庞大的客户群(美国、欧洲、日本)。
2017年TAKARA宝生物日本总部又收购了：美国Rubicon Genomics单细胞测序文库构建的领导品Pai，代表性产品之一为：Rubicon Genomics RB3050/R30050 PicoPLEX® WGA Kit 单细胞全基因组扩增试剂盒Whole Genome Amplification from Single Cells，ThruPLEX® Plasma-seq Kit，ThruPLEX® DNA-seq Kit，PicoPLEX® DNA-seq Kit，PicoPLEX® WGA Kit， TransPLEX® C-WTA Kit.
常见问题解答
1. ThruPLEX Plasma-seq可以使用什么类型的样品和输入？
ThruPLEX Plasma-seq Kit旨在从分离自血浆的无细胞DNA中产生Illumina NGS的DNA文库。 ThruPLEX Plasma-seq化学被重新制备，以产生具有广泛GC覆盖率的多种文库，提供1至30ng *无细胞DNA的可重复测序结果。
应用输入样品类型推荐输入量
血浆和液体活组织检查DNA测序; ctDNA分析，靶向测序从血浆中分离的无细胞DNA 1 ng - 30 ng *
库的输入量小于1 ng，超过30 ng。
2. ThruPLEX DNA-seq Kit和新型ThruPLEX Plasma-seq Kit之间的主要技术差异是什么？
ThruPLEX Plasma-seq Kit已经针对从血浆中分离的无细胞DNA进行了优化。
ThruPLEX Plasma-seq Kit不需要剪切或破碎无细胞DNA。
协议是不同的（见下文）。
3. ThruPLEX Plasma-seq和ThruPLEX DNA-seq之间的协议差异是甚么？
模板制备步骤：体积和保持温度
库的合成步骤：体积和培养温度
文库扩增步骤：扩增循环的体积和数量
4. 来自ThruPLEX Plasma-seq的试剂可以与ThruPLEX DNA-seq？
不，ThruPLEX Plasma-seq Kit的内容物与其他Rubicon Genomics产品不可互换。
5. 你有什么类型的索引或条形码？
ThruPLEX Plasma-seq 12S和48S试剂盒提供分布在管或96孔板中的与Illumina兼容的8nt单指数。这些索引序列由英国剑桥的Wellcome Trust Sanger研究所开发。关于Sanger指数序列的信息可以在Nature Methods 7,111-118（2010）中找到。
ThruPLEX Plasma-seq 96D试剂盒配备了分布在96孔板中的Illumina TruSeq HT i5和i7双重指标。 i5索引具有Illumina D501至D508序列，i7索引具有D701至D712序列。
有关索引的更多信息，请参阅“ThruPLEX Plasma-seq套件使用手册”中的附录1。
6. 您有什么建议将血浆中的无细胞DNA分离出来？
使用QIAamp®循环核酸试剂盒（Qiagen，CAT.NO.55114）制备的无细胞DNA优化ThruPLEX Plasma-seq。
含有输入DNA的缓冲液中Tris和EDTA的浓度分别不得超过10mM和0.1mM。避免含磷酸盐缓冲液。
7. 我应该使用什么样的收集管来吸取受试者的血液？
Rubicon Genomics的科学人员使用K2 EDTA（Becton Dickinson，CAT。NO。36643）在Vacutainer Venous Blood Collection Tubes中收集的血液。
8. 在分离无细胞DNA之前，我应该如何在血液中制备血浆？
使用双离心方案将血浆与血液分离。将处理过的血浆样品在-80°处储存，直到不分离DNA。
9. 从ThruPLEX Plasma-seq Kit中可以使用从尿液或其他生物流体中分离的无细胞DNA吗？
ThruPLEX Plasma-seq用从人血浆中分离的无细胞DNA进行了优化;然而，该试剂盒可能与来自其他生物流体的降解的DNA一起使用。必须首先用DNA提取/纯化试剂盒提取其他样品来源的无细胞DNA。由于来自这些来源的无细胞DNA通常被降解/分裂，因此不需要额外的碎裂。每个反应的无细胞输入DNA的体积不得超过10μL。
10. ThruPLEX Plasma-seq Kit是否与任何目标浓缩系统兼容？
是的，ThruPLEX Plasma-seq Kit与主要的exome和目标富集产品兼容，包括AgilentSureSelect®，RocheNimbleGen®SeqCap®EZ和定制面板。
红荣微再（上海）生物工程技术有限公司主营：干细胞、JZYL、细胞ZL、器官再生 四大板块的产品，以“传递科学价值，服务科学研究”为宗旨，经过近两年的努力推广，红荣微再（上海）生物工程技术有限公司在全ZG代理的CellArtis干细胞产品已经成功销往：上海市、北京市、重庆市、天津市、广东省的广州市、深圳市、珠海市、江西省的南昌市、四川省的成都市、绵阳、浙江省的杭州市、宁波、温州、江苏省的南京市、苏州、无锡、泰州、湖南省的长沙、陕西省的西安市、杨凌、甘肃省的兰州、宁夏的银川、新疆的乌鲁木齐、广西省的南宁市、山东省的济南、青岛、东北三省 辽宁省的沈阳市、大连市、吉林省的长春市、黑龙江省的哈尔滨市等ZG绝大部分省市和自治区。
欢迎您致电 华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司 手机号：152 1681 4001。华雅再生医学-客服QQ: 316 808 6348
FAQS
1. What types of samples ａnd inputs can be used with ThruPLEX Plasma-seq?
ThruPLEX Plasma-seq Kit is designed to generate DNA libraries for Illumina NGS from cell-free DNA isolated from plasma. The ThruPLEX Plasma-seq chemistry is reformulated to produce diverse libraries with broad GC coverage, providing reproducible sequencing results from 1 to 30 ng* of cell-free DNA.
ApplicationInput sample typeRecommended input amount
Sequencing of DNA from Plasma & Liquid Biopsy; ctDNA Analysis, Targeted SequencingCell-free DNA isolated from plasma1 ng – 30 ng*
*Libraries have been generated from input amounts less than 1 ng ａnd over 30 ng.
Contact technical support for further information.
2. What are the major technical differences between ThruPLEX DNA-seq Kit ａnd the new ThruPLEX Plasma-seq Kit?
ThruPLEX Plasma-seq Kit has been optimized for cell-free DNA isolated from plasma.
Shearing or fragmentation of the cell-free DNA is not necessary with ThruPLEX Plasma-seq Kit.
The protocol is different (see below).
3. What are the protocol differences between the ThruPLEX Plasma-seq ａnd ThruPLEX DNA-seq?
Template Preparation Step: volumes ａnd holding temperature
Library Synthesis Step: volumes ａnd incubation temperature
Library Amplification Step: volumes ａnd number of amplification cycles
4. Can the reagents from ThruPLEX Plasma-seq be interchanged with the reagents from ThruPLEX DNA-seq?
No, contents of ThruPLEX Plasma-seq Kit are not interchangeable with other Rubicon Genomics products.
5. What type of indexes or barcodes do you have available?
ThruPLEX Plasma-seq 12S ａnd 48S Kits are provided with Illumina-compatible 8nt single indexes dispensed in tubes or a 96-well plate. These index sequences were developed by the Wellcome Trust Sanger Institute in Cambridge, UK. Information about the Sanger index sequences can be found in Nature Methods 7, 111-118 (2010).
ThruPLEX Plasma-seq 96D Kit are provided with the Illumina TruSeq HT i5 ａnd i7 dual indexes dispensed in a 96-well plate. The i5 indexes have the Illumina D501 to D508 sequences, ａnd the i7 indexes have the D701 to D712 sequences.
For more information about the indexes, refer to Appendix 1 in the ThruPLEX Plasma-seq Kit Instruction Manual.
红荣微再（上海）生物工程技术有限公司主营：干细胞、JZYL、细胞ZL、器官再生 四大板块的产品，以“传递科学价值，服务科学研究”为宗旨，经过近两年的努力推广，红荣微再（上海）生物工程技术有限公司在全ZG代理的CellArtis干细胞产品已经成功销往：上海市、北京市、重庆市、天津市、广东省的广州市、深圳市、珠海市、江西省的南昌市、四川省的成都市、绵阳、浙江省的杭州市、宁波、温州、江苏省的南京市、苏州、无锡、泰州、湖南省的长沙、陕西省的西安市、杨凌、甘肃省的兰州、宁夏的银川、新疆的乌鲁木齐、广西省的南宁市、山东省的济南、青岛、东北三省 辽宁省的沈阳市、大连市、吉林省的长春市、黑龙江省的哈尔滨市等ZG绝大部分省市和自治区。
欢迎您致电 华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司 手机号：152 1681 4001。华雅再生医学-客服QQ: 316 808 6348
6. What recommendations do you have for isolating the cell-free DNA from plasma?
ThruPLEX Plasma-seq was optimized with cell-free DNA prepared using the QIAamp® Circulating Nucleic Acid Kit (Qiagen, CAT. NO. 55114).
The concentrations of Tris ａnd EDTA in the buffer containing the input DNA must not exceed 10 mM ａnd 0.1 mM, respectively. Avoid phosphate containing buffers.
7. What kind of collection tube should I use to draw blood from subjects?
The scientific staff at Rubicon Genomics uses blood collected in Vacutainer Venous blood Collection Tubes with K2 EDTA (Becton Dickinson, CAT. NO. 36643).
For detailed instructions, refer to the Blood Collection ａnd Plasma Preparation Protocol at the ThruPLEX Plasma-seq Product Page, under the Resources tab: http://rubicongenomics.com/products/thruplex-plasma-seq-kit/.
8. How should I prepare plasma from blood before isolating the cell-free DNA?
Plasma should be separated from blood using a double-centrifugation protocol. Store processed plasma samples at –80° until cell-free DNA is to be isolated.
For detailed instructions, refer to the Blood Collection ａnd Plasma Preparation Protocol at the ThruPLEX Plasma-seq Product Page, under the Resources tab: http://rubicongenomics.com/products/thruplex-plasma-seq-kit/.
9. Can cell-free DNA isolated from urine or other biofluids be used with ThruPLEX Plasma-seq Kit?
ThruPLEX Plasma-seq was optimized with cell-free DNA isolated from human plasma; however, the kit may work with degraded DNA from other biofluids. Cell-free DNA from other sample sources must first be extracted with a DNA extraction/purification kit. Since cell-free DNA from these sources is normally degraded/fragmented, additional fragmentation is unnecessary. The volume of input cell-free DNA for each reaction must not exceed 10 µL.
10. Is ThruPLEX Plasma-seq Kit compatible with any target enrichment systems?
Yes, ThruPLEX Plasma-seq Kit is compatible with the major exome ａnd target enrichment products, including Agilent SureSelect®, Roche NimbleGen® SeqCap® EZ ａnd custom panels. ThruPLEX Plasma-seq Kit target enrichment protocols ａnd application notes can be accessed at: http://rubicongenomics.com/applications/enrichment/.
红荣微再（上海）生物工程技术有限公司主营：干细胞、JZYL、细胞ZL、器官再生 四大板块的产品，以“传递科学价值，服务科学研究”为宗旨，经过近两年的努力推广，红荣微再（上海）生物工程技术有限公司在全ZG代理的CellArtis干细胞产品已经成功销往：上海市、北京市、重庆市、天津市、广东省的广州市、深圳市、珠海市、江西省的南昌市、四川省的成都市、绵阳、浙江省的杭州市、宁波、温州、江苏省的南京市、苏州、无锡、泰州、湖南省的长沙、陕西省的西安市、杨凌、甘肃省的兰州、宁夏的银川、新疆的乌鲁木齐、广西省的南宁市、山东省的济南、青岛、东北三省 辽宁省的沈阳市、大连市、吉林省的长春市、黑龙江省的哈尔滨市等ZG绝大部分省市和自治区。
欢迎您致电 华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司 手机号：152 1681 4001。华雅再生医学-客服QQ: 316 808 6348
