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A0086 SEPARASE (Phospho-Ser801) Antibody

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A0086  SEPARASE (Phospho-Ser801) Antibody

Reactivity Applications MW. Source
H, M WB IHC IF ELISA 233 kDa Rabbit
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.

Product Name SEPARASE (Phospho-Ser801) Antibody
Synonyms ESP1; SEPARIN, EXTRA SPINDLE POLES-LIKE 1, extra spindle poles like 1 (S. cerevisiae)
Immunogen The antiserum was produced against synthesized peptide derived from human SEPARASE around the phosphorylation site of Ser801.
Immunogen Region Internal
Clonality Polyclonal
Modification sites H:S801 M:S1189
Application dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
Specificity SEPARASE (Phospho-Ser801) Antibody detects endogenous levels of SEPARASE only when phosphorylated at serine801.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Physical Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration 1 mg/ml
Storage/Stability Store at -20℃/1 year
 

NCBI Gene Symbol ESPL1
Database Links
Application images

Immunofluorescence analysis of HuvEc cells, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from 293 cells treated with EGF 200ng/ml 30', using SEPARASE (Phospho-Ser801) Antibody. The lane on the right is treated with the synthesized peptide.

 

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