胶质细胞系来源神经营养因子(GDNF)重组蛋白Recombinant Glial Cell Line Derived Neurotrophic Factor (GDNF)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Rattus norvegicus (Rat) |
| Product No. | XY043Ra01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 16.2kDa |
| Concentration | 9.4 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Formulation | Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, ａnd preservative. |
SEQUENCES
胶质细胞系来源神经营养因子(GDNF)重组蛋白The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG S- SPD KQAAALPRRE RNRQAAAASP ENSRGKGRRG QRGKNRGCVL TAIHLNVTDL GLGYETKEEL IFRYCSGSCE AAETMYDKIL KNLSRSRRLT SDKVGQACCR PVAFDDDLSF LDDSLVYHIL RKHSAKRCGC I
USAGE
胶质细胞系来源神经营养因子(GDNF)重组蛋白Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot ａnd store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, ａnd no obvious degradation ａnd precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa ａnd 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location ａnd size approximation of proteins of interest quick ａnd easy.
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