美国人RXR alpha可诱导表达稳定细胞株

美国PrimCells诱导性稳定细胞株： PrimCells建立的独有的诱导性细胞GX开发系统，能在极短时间内筛选出低泄漏，高诱导性的稳定细胞株，成功开发出一系列核受体诱导表达的稳定细胞株产品，为多家知名制药公司研发部门和科研院所提供产品与服务，广受用户好评。针对客户的特定需求，PrimCells亦提供量身定制服务，通过密切的客户交流和GX的筛选体系，严格保证客户在3-4周内即获得高质量的诱导性稳定细胞株，具有市场其他供应商无可比拟的时效性。
Nuclear hormone receptors (NHRs) are a superfamily of transcription factors that function as powerful metabolic regulators to control a variety of systemic processes in physiology. They also play key roles in the pathophysiology of many major disease states, such as diabetes, obesity, inflammation, atherosclerosis ａnd heart failure. To date, this superfamily has provided a rich source of drug design targets ａnd it is continuing to be one of the hottest areas for pharmaceutical researches.

PrimCells now provides a comprehensive set of NHR expressing cell lines to the research community. These high-quality, flag-and/or HA-tagged NHR-expressing cell lines aim to facilitate further biochemical ａnd molecular studies of their functions ａnd hence encourage new strategies for drug design.

The human VDR stable cell line was created by stable co-transfection of HEK293 cells with two plasmids expressing the bacterial Tet repressor (TetR) ａnd the human VDR protein with HA tag, respectively. Since the human VDR gene is driven by a promoter containing two tetracycline operator (TeTO2) sites, its expression can be induced by doxycycline.