Prospec PRO-567 Haptoglobin Human/重组人结合珠蛋白
- 产地：美洲
- 供应商：厦门慧嘉生物科技有限公司
- 供应商报价：电议
- 标签：Prospec PRO-567 Haptoglobin Human/重组人结合珠蛋白,生物试剂,生化试剂,供应Prospec PRO-567 Haptoglobin Human/重组人结合珠蛋白,厦门慧嘉生物科技有限公司
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| Synonyms | Haptoglobin, HP, BP, HPA1S, MGC111141, HP2-ALPHA-2. |
| Introduction | Haptoglobin is a glycoprotein which is synthesized in the liver ａnd circulates in the blood. Haptoglobin is produced typically by hepatocytes but also by other tissues: e.g. skin, lung, ａnd kidney. It is a positive acute phase protein that binds free hemoglobin ａnd removes it from the circulation to prevent kidney injury, ａnd iron loss following hemolysis. The haptoglobin-hemoglobin complex is subsequently removed by the reticuloendothelial system （generally the spleen）. As the reticuloendothelial system removes the haptoglobin-hemoglobin complex from the body, haptoglobin levels are reduced in hemolytic anaemias. In the course of binding hemoglobin, haptoglobin sequesters the iron inside hemoglobin, preventing iron-utilizing bacteria from benefitting from hemolysis. Haptoglobin consists of two ?- ａnd two ?-chains, connected by disulfide bonds. Three major haptoglobin phenotypes are known to exist （Hp 1-1, Hp 2-1, ａnd Hp 2-2）. Hp 1-1 is biologically the most effective in binding free hemoglobin ａnd suppressing inflammatory responses associated with free hemoglobin. Hp 2-2 is biologically the least active, ａnd Hp 2-1 is moderately active. Haptoglobin’s molecular mass ranges from 8-200 kDa. Reduced levels can be seen in haemolysis ａnd impaired liver function. High levels are a marker for acute or chronic inflammation. Ahaptoglobinemia or hypohaptoglobinemia are caused by mutations in the haptoglobin gene and/or its regulatory regions. Haptoglobin is also linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn＇s disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson＇s disease, ａnd a reduced incidence of Plasmodium falciparum malaria. |
| Description | Haptoglobin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing （aa. 145-405） fusion protein with His tag ａnd having a total Mw of 33 kDa （4 kDa His-tag）. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. |
| Formulation | Each mg was lyophilized with 1xPBS, 0.1% SDS ａnd 1mM DTT. |
| Solubility | It is recommended to reconstitute the lyophilized Haptoglobin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Haptoglobin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Haptoglobin should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. |
| Applications | 1. Positive control for Western blot 2. Antibody production 3. Protein assay. |
| Purity | Greater than 90.0% as determined by SDS-PAGE ａnd HPLC. |
| Usage | Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
