Prospec PRO-539 LBP Mouse/重组小鼠脂多糖结合蛋白
- 产地：美洲
- 供应商：厦门慧嘉生物科技有限公司
- 供应商报价：电议
- 标签：Prospec PRO-539 LBP Mouse/重组小鼠脂多糖结合蛋白,生物试剂,生化试剂,供应Prospec PRO-539 LBP Mouse/重组小鼠脂多糖结合蛋白,厦门慧嘉生物科技有限公司
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| Synonyms | Lipopolysaccharide-binding protein, LBP, Ly88. |
| Introduction | Lipopolysaccharides （LPS） are a type of glycolipids on the outer cell wall of Gram-negative bacteria. Lipopolysaccharide binding protein （aka LBP） is a plasma protein which facilitates the diffusion of bacterial LPS （endotoxin）. LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In cooperation with bactericidal permeability-increasing protein （BPI）, LBP binds LPS ａnd interacts with the CD14 receptor, most likely playing a role in regulating LPS-dependent monocyte responses. LBP belongs to a family of structurally ａnd functionally related proteins, including BPI, plasma cholesteryl ester transfer protein （CETP）, ａnd phospholipid transfer protein （PLTP）. The LBP gene is found on chromosome 20, directly downstream of the BPI gene. LBP catalyzes the transfer of LPS monomers from LPS aggregates to HDL particles, to phospholipid bilayers, ａnd to a binding site on soluble CD14 （sCD14）. sCD14 is capable of speeding up the transfer by receiving an LPS monomer from an LPS aggregate, ａnd then yielding it to an HDL particle, therefore acting as a soluble "shuttle" for an insoluble lipid. |
| Description | LBP is produced from mouse LBP transfected CHO-cells in serum free medium. For transfection we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before tranfection the complete mouse LBP cDNA was amplified by PCR ａnd cloned into expression vector p-POL-DHFR. RmLBP was produced by his-tag by means of metal affinity purification with Talon ａnd controlled by SDS page. Attention: His-tag at the c-terminal end of the LBP has no protease site ａnd is not to split off. up to 2μg/ml LBP mediates binding of FITC-LPS （0.5μg/ml） to CD14+CHO transfectants （2 x 1,000,000/ml）. |
| Source | Chinese Hamster Ovarian Cells （CHO）. |
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. |
| Formulation | Recombinant Mouse LBP was lyophilized from a protein solution （1 mg/ml） containing phosphate-buffered saline, pH 7.2. |
| Solubility | It is recommended to reconstitute the lyophilized LBP in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized LBP Mouse Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LBP should be stored at 4°C between 2-7 days ａnd for future use below -18°C. Please prevent freeze-thaw cycles. |
| Applications | ? ELISA ? Western Blotting. |
| characterization | On SDS-PAGE commassie blue stained gel, the 95% purified recombinant protein shows a band at 58 kDa. |
| Usage | Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
