Anti-BTK,鼠抗人非受体型酪氨酸激酶BTK单克隆抗体

• Product nameAnti-BTK antibody [7F12H4,6G5F6]See all BTK primary antibodies ...
• Description
  Mouse monoclonal [7F12H4,6G5F6] to BTK
• Tested applicationsFlow Cyt, WB, ELISA, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Human
• Immunogen

  Ni-NTA purified recombinant BTK fragment （Human） expressed in E.coli strain BL21 （DE3）

• Positive controlK562 cell lysate （WB）, Human Lymph node tissue （IHC-P）, Human Lymph follicle tissue （IHC-P）, Jurkat cells （ICC/IF）.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot ａnd store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: Ascites
•  
• PurityAscites
• ClonalityMonoclonal
• Clone number7F12H4,6G5F6
• IsotypeIgG1
• Research Areas
  • Signal Transduction
   
  • Protein Phosphorylation
   
  • Tyrosine Kinases
  • Other

  • Immunology
   
  • Innate Immunity
   
  • TLR Signaling

Applications

Target

• FunctionPlays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes.
• Involvement in diseaseDefects in BTK are the cause of X-linked agammaglobulinemia （XLA） [MIM:300755]; also known as X-linked agammaglobulinemia type 1 （AGMX1） or immunodeficiency type 1 （IMD1）. XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes ａnd leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.
  Defects in BTK may be the cause of X-linked hypogammaglobulinemia ａnd isolated growth hormone deficiency （XLA-IGHD） [MIM:307200]; also known as agammaglobulinemia ａnd isolated growth hormone deficiency or Fleisher syndrome or isolated growth hormone deficiency type 3 （IGHD3）. In rare cases XLA is inherited together with isolated growth hormone deficiency （IGHD）.
• Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
  Contains 1 Btk-type zinc finger.
  Contains 1 PH domain.
  Contains 1 protein kinase domain.
  Contains 1 SH2 domain.
  Contains 1 SH3 domain.
• Post-translational
  modificationsAutophosphorylated on Tyr-223 ａnd Tyr-551. Phosphorylation of Tyr-223 may create a docking site for a SH2 containing protein.
• Cellular localizationCytoplasm. Membrane. Nucleus.
• Information by UniProt
• Database links
  • Entrez Gene: 695 Human
  • Omim: 300300 Human
  • SwissProt: Q06187 Human
  • Unigene: 159494 Human
• Alternative names
  • Agammaglobulinaemia tyrosine kinase antibody
  • AGMX 1 antibody
  • AGMX1 antibody

  see all

Anti-BTK antibody [7F12H4,6G5F6] images

• 

  Western blot - BTK antibody [7F12H4,6G5F6] （ab54219）
  All lanes : Anti-BTK antibody [7F12H4,6G5F6] （ab54219） at 1/500 dilution
  
  Lane 1 : Molecular weight marker
  Lane 2 : Truncated BTK recombinant protein
  Lane 3 : K562 cell lysate
  
  
  Predicted band size : 76 kDa
  Observed band size : 80 kDa （why is the actual band size different from the predicted?）
  
• 

  Immunohistochemistry （Paraffin-embedded sections） - BTK antibody [7F12H4,6G5F6] （ab54219）
  Immunohistochemical analysis of paraffin embedded human lymph node tissue, showing cytoplasmic ａnd membrane localization, using ab54219 （1/500 dilution） with DAB staining.
• 

  Immunohistochemistry （Paraffin-embedded sections） - BTK antibody [7F12H4,6G5F6] （ab54219）
  Immunohistochemical analysis of paraffin embedded human lymph follicle tissue, showing cytoplasmic ａnd membrane localization, using ab54219 （1/500 dilution） with DAB staining.
• 

  Immunocytochemistry/ Immunofluorescence - BTK antibody [7F12H4,6G5F6] （ab54219）
  Immunofluorescence analysis of Jurkat cells using ab54219 with FITC-IgG.
• 

  Flow Cytometry-BTK antibody [7F12H4,6G5F6]（ab54219）
  Overlay histogram showing Jurkat cells stained with ab54219 （red line）. The cells were fixed with methanol （5 min） ａnd then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody （ab54219, 1/50 dilution） for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG （H+L） （ab96879） at 1/500 dilution for 30 min at 22°C. Isotype control antibody （black line） was mouse IgG1 [ICIGG1] （ab91353, 2µg/1x10⁶ cells） used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde （10 min）/permeabilized in 0.1% PBS-Tween used under the same conditions.
  
  

References for Anti-BTK antibody [7F12H4,6G5F6] （ab54219）