SNAP25 C.elegans/重组神经突触关联蛋白25kDa C.elegans
- 产地：美洲
- 供应商：厦门慧嘉生物科技有限公司
- 供应商报价：电议
- 标签：SNAP25 C.elegans/重组神经突触关联蛋白25kDa C.elegans,生物试剂,生化试剂,供应SNAP25 C.elegans/重组神经突触关联蛋白25kDa C.elegans,厦门慧嘉生物科技有限公司
产品库
| Synonyms | Super-Protein, SUP, RIC4, SEC9, SNAP, RIC-4, SNAP25, SNAP-25, Synaptosomal-associated protein 25, Synaptosomal-associated 25 kDa protein, FLJ23079, bA416N4.2, dJ1068F16.2. |
| Introduction | Synaptic vesicle membrane docking ａnd fusion is mediated by SNAREs （soluble N-ethylmaleimide-sensitive factor attachment protein receptors） located on the vesicle membrane （v-SNAREs） ａnd the target membrane （t-SNAREs）. The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE ａnd the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix ａnd the protein encoded by this gene contributes the other two. Therefore, SNAP25 product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. The synaptosomal-associated protein （SNAP-25） is an essential component of the core complex that mediates presynaptic vesicle trafficking. Thus, SNAP-25 is directly involved in the release of neurotransmitters. |
| Description | Recombinant C.elegans SNAP-25 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 207 amino acids ａnd having a molecular mass of 23kDa. SNAP-25 gene was amplified by PCR from C.elegans ａnd cloned into an E. coli expression vector. SNAP-25 was purified by using conventional chromatography techniques. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile filtered colorless solution. |
| Formulation | The protein contains 20mM Tris-HCl pH7.5, 50mM NaCl, 5mMDTT, 1mM EDTA ａnd 10% Glycerol. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Avoid multiple freeze-thaw cycles. |
| Purity | Greater than 95.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. |
| Sequence | MSGDDDIPEG LEAINLKMNA TTDDSLESTR RMLALCEESK EAGIKTLVML DDQGEQLERCEGALDTINQD MKEAEDHLKG MEKCCGLCVL PWNKTDDFEK TEFAKAWKKD DDGGVISDQPRITVGDSSMG PQGGYITKIT NDAREDEMDE NVQQVSTMVG NLRNMAIDMS TEVSNQNRQL DRIHDKAQSN EVRVESANKR AKNLITK. |
| Usage | Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
