Synonyms
Growth-regulated protein alpha, CXCL1, Melanoma growth stimulatory activity, MGSA, Neutrophil-activating protein 3, NAP-3, GRO-alpha（1-73）, chemokine （C-X-C motif） ligand 1, GRO1, GROa, SCYB1, MGSA-a, MGSA alpha.
Introduction
Chemokine （C-X-C motif） ligand 1 （CXCL1） is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, Neutrophil-activating protein 3 （NAP-3） ａnd melanoma growth stimulating activity, alpha （MSGA-α）. It is secreted by human melanoma cells, has mitogenic properties ａnd is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils ａnd epithelial cells, ａnd has neutrophil chemoattractant activity. CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors ａnd is involved in the processes of angiogenesis, inflammation, wound healing, ａnd tumorigenesis. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines.
Description
CXCL1 Rat Recombinant produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 73 amino acids ａnd having a molecular mass of 7.8 kDa.
The Rat CXCL1 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearence
Sterile Filtered White lyophilized （freeze-dried） powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated （1mg/ml） solution in 20mM PB, pH 7.4, ａnd 150mM NaCl.
Solubility
Lyophilized CXCL1 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution CXCL1 should be stored at 4C between 2-7 days ａnd for future use below -18C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.
Purity
Greater than 97.0% as determined by:
（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE.
Amino Acid Sequence
APVANELRCQ CLQTVAGIHF KNIQSLKVMP PGPHCTQTEV IATLKNGREA
CLDPEAPMVQ KIVQKMLKGV PK.
Biological Activity
Determined by its ability to chemoattract rat neutrophils using a concentration range of 10-100ng/ml.
Usage
Prospec＇＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
