PCR核酸试剂 水泡疹病毒1/2型检测试剂盒（PCR荧光探针法）

PCR核酸试剂 水泡疹病毒1/2型检测试剂盒(PCR荧光探针法)

广州健仑生物科技有限公司

准备使用lyo master mix（8孔条）检测单纯疱疹病毒1和2型（HSV1＆2），水痘 - 带状疱疹病毒（VZV）和内部对照。
Ready to use lyo master mix (8-well strips) for detection of herpes simplex virus 1 & 2 (HSV1&2), varicella-zoster virus (VZV) and internal control

PCR核酸试剂 水泡疹病毒1/2型检测试剂盒(PCR荧光探针法)

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PCR核酸试剂 水泡疹病毒1/2型检测试剂盒(PCR荧光探针法)

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

（三）实验器材
(1)活材料：大肠杆菌(E.coli)。
(2)培养基和试剂：牛肉蛋白胨液体培养基14支(每支10mL)，浓缩5 倍的牛肉蛋白胨培养基1支。无菌酸溶液(甲酸：乙酸：乳酸=3：1：1)。
(3)器材：1mL无菌吸管、摇床、冰箱、光电比色计、标签等。
（四）实验方法
方法(1)
1)接种：取13支装有牛肉蛋白胨培养液的试管，贴上标签(注明菌名、培养处理、培养时间、组号)。按无菌操作法用吸管向每管准确加入0.2mL的大肠杆菌培养液，接种后，轻轻摇荡，使菌体混匀。另一支不接种的培养管注明CK(对照)。
2)培养：将接种后的培养管置于摇床上，在37℃下振荡培养。其中9支培养管分别于培养的0、1．5、3、4、6、8、10、12和14h后取山，放冰箱中贮存，待测定。加酸处理。取出经4h培养的另二支培养管，按无菌操作法加入lmL无菌酸溶液，摇匀后放回摇床上，继续振荡培养，于培养8h和14h后取出放冰箱中贮存，待测定。加富营养物处理。余下的二支培养管于培养6h后取出，按无菌操作法加入浓缩5倍的牛肉蛋白胨培养液1mL，摇匀后，继续进行振荡培养，于培养8h和14h后取出，放入冰箱中贮存，待测定。
3)比浊：将培养不同时间、形成不同细胞浓度的细菌培养液进行适当稀释，使光密度值在0.0-0.4范围内，以未接种的牛肉蛋白胨液体培养基调零点，在光电比色计上，选用400-440nm波长的滤光片进行比浊，从Z稀浓度的菌悬液开始，依次测定。
方法
将大肠杆菌接入装有牛肉蛋白胨培养液的小试管中(试管要能插入放比色杯的比色槽内)。37℃下振荡培养，分别在0、1.5 、3、4、6、8、10、12和14h取出，以未接种培养液调零点，在光电比色计上比色。比色时应自制一个暗盒将培养管和比色槽罩住，以形成一个暗室。

(C) experimental equipment
(1) Live material: E. coli.
(2) Culture medium and reagents: beef peptone liquid culture medium 14 (each 10mL), concentrated five times the beef peptone medium 1 branch. Sterile acid solution (formic acid: acetic acid: lactic acid = 3: 1: 1).
(3) equipment: 1mL sterile pipette, shaker, refrigerator, photoelectric colorimeter, labels and so on.
(D) experimental methods
method 1)
1) Inoculation: Take 13 test tubes containing beef peptone culture solution and label them (note the fungus name, culture treatment, culture time and group number). Press aseptically using a pipette to each tube accuray add 0.2mL of E. coli culture medium, inoculation, gently shaking, the bacteria mix. Another uninoculated culture tube was labeled CK (control).
2) Culture: The inoculated culture tube was placed on a shaker and cultured with shaking at 37 ° C. Nine of the culture tubes were collected at 0, 1.5, 3, 4, 6, 8, 10, 12 and 14 h after cultivation respectively and stored in the freezer for determination. Acid treatment. Remove the 4h culture of the other two culture tubes, according to aseptic method by adding lmL aseptic acid solution, shake back into the shaker, continue shaking culture, and cultured in the 8h and 14h after the refrigerator out of storage, to be determined. Enriched with nutrients. The remaining two culture tubes were removed 6h after culture, according to aseptic method by adding 5 times concentrated beef peptone broth 1mL, shake, continue shaking culture, after the culture 8h and 14h removed, into the refrigerator storage , To be determined.
3) turbid: the culture at different times, the formation of different cell concentrations of bacterial culture medium appropriate dilution, the optical density value in the range of 0.0-0.4 to uninoculated beef peptone liquid medium zero point on a photoelectric colorimeter , The choice of 400-440nm wavelength filter turbidity, the most dilute concentration of bacterial suspension, followed by determination.
method
Connect E. coli into a small test tube containing beef peptone culture (the test tube can be inserted into the cuvette). The cells were shaken at 37 ° C and taken out at 0, 1.5, 3, 4, 6, 8, 10, 12 and 14 h, respectively. Colorimetric should be made of a cassette culture tube and colorimetric tank cover to form a darkroom.