Mouse Anti-ssDNA IgG-specific ELISA...

SpeciesReactivity
Mouse

Form
Stds= 0-10,000 ng/ml; Sample: 100 ul (1:100); Sensitivity; 300 ng/ml; 75 min assay; Quantitative; Ag-based Ab assay, 96 tests

Samples
Serum. Plasma, Culture medium and other fluids may be adapted

KitContents
1. Purified ssDNA Coated strip plate (8 wells x 12 strips) 5321 2. Mouse Anti-ssDNA IgG std A (0 ug/ml), 0.65 ml 5322 3. Mouse Anti-ssDNA IgG std B (0.31 ug/ml) 0.65 ml 5323 4. Mouse Anti-ssDNA IgG std C (0.62 ug/ml).0.65 ml 5324 5. Mouse Anti-ssDNA IgG std D (1.25 ug/ml) 0.65 ml 5325 6. Mouse Anti-ssDNA IgG std E (2.5 ug/ml) 0.65 ml 5326 7. Mouse Anti-ssDNA IgG std F (5 ug/ml) 0.65 ml 5327 8. Goat Anti-mouse IgG-HRP conjugate , 9. 0.120 ml 5328 10. Sample Diluent, (10x), 10ml SD-10 11. Wash Solution (100X), 10 ml WB-100 12. TMB Substrate, 12 ml 80091 13. Stop solution, 12 ml 80101 14. Complete Instruction Manual. M-5320

SpCrossreactivity
The antigen used for coating is ssDNA. Many antibodies to DNA react with either ssDNA or dsDNA or both. This kit will measure anti-ssDNA. The detection antibodies used in the kit are directed against mouse IgG (heavy chain) and specific for IgG. The antibodies do not react with IgM, IgA or other isotypes. However, they may react with several IgG isotypes (IgG1, IgG2 etc.). ADI has other kits for Ig?s total (5310-Ig?s) and IgM (5330-IgM specific).

MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates

For research use only, not for diagnostic or therapeutic use.