Human Transferrin （Tf） ELISA Kit

SpeciesReactivity
Human

Form
Stds=20-400 ng/ml ; Sample=100 ul (diluted); Sensitivity= 10 ng/ml; 105 min assay; Quantitative, Sandwich, 96 tests

Samples
Serum, Plasma, Urine, Culture medium

KitContents
1. Goat Anti-Human Transferrin coated strip plate (8 wells x 12 strips) 1211 2. Human Transferrin Std. A (0 ng/ml), 0.25 ml 1212 3. Human Transferrin Std B (50 ng/ml), 0.25 ml 1213 4. Human Transferrin Std C (100 ng/ml), 0. 25 ml 1214 5. Human Transferrin Std D (250 ng/ml), 0. 25 ml 1215 6. Human Transferrin Std E (500 ng/ml), 0. 25 ml 1216 7. Human Transferrin Std F (800 ng/ml), 0. 25 ml 1217 8. (20x) Sample Diluent, 10ml SD-20 9. Wash Buffer (100X), 10 ml WB-100 10. 100x Anti-Human Transferrin-HRP Conjugate, 0.12 ml 1219 11. TMB Substrate, 12 ml 80091 12. Stop solution, 12 ml 80101 13. Instruction Manual M-1210

SpCrossreactivity
The antibodies used in this kit are specific to transferrin, with no significant reactivity with any other human serum proteins. Sera from rhesus and cynomolgous monkeys, baboons and chimpanzees showed extensive reactivity in the ELISA, and serum dilution curves were linear. Therefore, ADI?s human Transferrin ELISA kit may be adapted to measure Transferrin in rhesus and cynomolgous monkeys, baboons and chimpanzees. Serum Transferrin from the following species showed no significant reactivity at 1:100 serum dilutions: mouse, rat, hamster, guinea pig, bovine, pig, sheep, rabbit or chicken.

MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates

For research use only, not for diagnostic or therapeutic use.