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ProSwift RP Protein Column

产品信息
Monolith Reversed-Phase Columns for High-Resolution Protein Separations at High Flow Rates

ProSwift® reversed-phase monolith columns uniquely provide the advantages of high resolution at exceptionally high flow rates for protein separations. They deliver the outstanding resolving power of nonporous analytical media combined with faster separations than any bead based columns available. ProSwift polymer monolith columns achieve long column lifetime, exhibit reproducibility even after hundreds of runs, and have broad pH stability, providing high stringent wash capability.

  • High resolution at high speed

  • Highest operational flow rates available

  • High throughput and improved productivity

  • Optimal performance in a broad range of applications

  • Excellent stability over a wide pH range of 1 to 14

  • Outstanding reproducibility and ruggedness

  • High stringent wash compatible, for example, 1 M NaOH

  • High loading capacity

ProSwift polymer monolith media are uniquely suited for the separation of proteins. Each monolith is a single cylindrical polymer rod containing an uninterrupted, interconnected network of flow-through channels of a specific pore size. These large channels and the monolith’s nonporous surfaces result in fast mass transfer for high-resolution and fast protein separations. The channels also produce low backpressure, allowing the use of higher linear velocities with minimal loss of resolution.

ProSwift Reversed-Phase Monolith Columns Specifications
Column NameProswift RP-15Proswift RP-2HProSwift RP-3U

Proswift RP-4H

ProSwift RP-10R
Column Dimensions4.6 × 50 mm4.6 × 50 mm4.6 × 50 mm1 × 50 mm1 × 250 mm1 × 50 mm
Base Matrix Material

Polystyrene-divinlbenzene

Surface Chemistry

Phenyl

Protein Binding
Capacity per mL of Polymer
3.0 mg/mL Lysozyme1.0 mg/ml Lysozyme.05 mg/ml Lysozyme2.3 mg/ml Lysozymen.d.*
Protein binding capacity per column2.2 mg Lysozyme0.8 mg Lysozyme0.3 mg Lysozyme0.1 mg Lysozyme0.5 mg Lysozymen.d.
Bed Height46 mm44 mm40 mm50 mm250 mm50 mm
Bed Volume (mL)0.76 mL0.73 mL0.65 mL0.039 mL0.196 mL0.039 mL
pH Range

1–14

1–10
Recommended Flow Rate0.5–4 mL/min1–10 mL/min1–16 mL/min0.1–0.4 mL/min0.1–0.3 mL/min40–60 µL/min
Maximum Flow Rate5 mL/min14 mL/min20 mL/min0.5 mL/min0.4 mL/min100 µL/min
Maximum Pressure

2800 psi
19.3 MPa

<2000 psi
13.8 Mpa
3000 psi
20.7 MPa
4350 psi
30 MPa
Operating Pressure

< 1500 psi
10.4 MPa

< 2500 psi
17.5 MPa
2900 psi
20 MPa
Temperature

70 °C

80 °C
Solvent Compatability

Most Common organic solvents

*n.d.=not determined

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