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glyclotech31-010 Glycotech 细胞流体剪切应力培养流动小室

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Parallel Plate Flow Chambers  

 

Human Neutrophils Rolling on
Human Endothelial Cells Expressing E-selectin

Parallel plate flow chambers are used in dynamic studies of cell adhesion under well-defined shear forces. Various gasket sizes allow flexibility in experimental design to operate with limited quantities of cells and/or reagents.

(Refer to Protocol 7)

Circular Flow Chamber (for 35mm tissue culture dishes)

31-001

Circular Flow Chamber Kit   

Technical Reference: Improvements to Parallel Plate Flow Chambers to Reduce Reagents and Cellular Requirements, Brown and Larson, BMC Immunology 2001, 2:9

 

Flow Chamber Kit Includes:

Two flow decks
(cast acrylic with different inlet/outlet designs)

8 silicon rubber gaskets (two of each size)
Flow Width = 0.25 cm, Thickness = 0.005 in
Flow Width = 0.25 cm, Thickness = 0.010 in
Flow Width = 0.50 cm, Thickness = 0.010 in
Flow Width = 1.00 cm, Thickness = 0.010 in

24 polypropylene tubing connectors (six of each)
Threaded 1/16 in. straight and L-connectors
Male and Female taper luer to 1/16 in. connectors

Silastic� laboratory tubing (1/16 in. ID x 10 ft.)
 

Replacement Gaskets and Coverslips for the Circular Flow Chamber
31-002Four Gaskets  (Flow Width = 0.25 cm, Thickness = 0.005 in)$30.00

 

31-003Four Gaskets  (Flow Width = 0.25 cm, Thickness = 0.010 in)$30.00

 

31-004Four Gaskets  (Flow Width = 0.50 cm, Thickness = 0.010 in)$30.00

 

31-005Four Gaskets  (Flow Width = 1.00 cm, Thickness = 0.010 in)$30.00

 

31-008Circular Glass Coverslips (pack of 30, 35 mm, #2)$30.00

 

 

Rectangular Flow Chamber (for standard microscope slide)

31-010

Rectangular Flow Chamber Kit

    

 

 

Flow Chamber Kit Includes:

One flow deck (cast acrylic)

4 silicon rubber gaskets (two of each size)
Flow Width = 1.00 cm, Thickness = 0.005 in
Flow Width = 1.00 cm, Thickness = 0.010 in

24 polypropylene tubing connectors (six of each)
Threaded 1/16 in. straight and L-connectors
Male and Female taper luer to 1/16 in. connectors

Silastic� laboratory tubing (1/16 in. ID x 10 ft.)
 

Replacement Gaskets for the Rectangular Flow Chamber
31-011Four Gaskets  (Flow Width = 1.0 cm, Thickness = 0.005 in) 

 

31-012Four Gaskets  (Flow Width = 1.0 cm, Thickness = 0.010 in) 

 

 

 

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