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美国Biotium原装进口钙离子指示剂/探针 美国Biotium原装进口钙离子指示剂/探针

产品信息
Fluo-3
Fluo-3 absorbs at 506 nm and emits at 526 nm when bound to calcium. It is one of the most widely used fluorescent indicators because it can be optimally excited by the commonlyused 488 argon-ion laser line and its relatively long wavelength emission reduces interference from background fluorescence. Unlike Fura-2 and Indo-1, neither the excitation nor the emission maximum of the dye shifts significantly before and after Ca2+ binding. As a result, the fluorometric ratioing technique is not applicable to Fluo-3. Fluo-3 is essentially nonfluorescent without Ca2+ present, but the fluorescence increases at least 40 times on Ca2+ binding. Because Fluo-3 binds Ca2+ more weakly (higher Kd) than Fura-2 and Indo-1, it is more useful for measuring high transient Ca2+ concentration during Ca2+ spikes.
Like other AM esters, Fluo-3 AM ester is relatively unstable when frequently exposed to moisture. For this reason, Biotium offers Fluo-3 AM ester in several packaging sizes including 2050µg and 10100µg, which facilitate storage and handling. Biotium has developed a unique purification procedure to ensure that the quality of our Fluo-3 AM is among the best (95% by HPLC) while maintaining very competitive prices.

Cat#


Product Name



Unit Size



Unit Price



59100



Calcium Calibration Buffer Kit (zero and 10 mM CaEGTA, 2x50mL)



kit



¥1470



90082



DMSO, anhydrous (Dimethyl sulfoxide, or  methyl sulfoxide)



10mL



¥199.92



59002



EDC (EDAC; 1-(3-dimethylaminopropyl-3-ethylcarbodiimide, hydrochloride))



100mg



¥776.16



50014



Fluo-3, AM ester



1mg



¥1728.72



50013



Fluo-3, AM ester



10100ug



¥1975.68



50016



Fluo-3, AM ester (special packaging)



2050ug



¥2410.8



50015



Fluo-3, AM ester, 1mM in anhydrous DMSO



1.0mL



¥1975.68



50010



Fluo-3, pentaammonium salt



1mg



¥1528.8



50011



Fluo-3, pentapotassium salt



1mg



¥1528.8



50012



Fluo-3, pentasodium salt



1mg



¥1528.8



59007



Ionomycin,calcium salt



1 mg



¥1552.32



59000



Pluronic F-127



1g



¥0



59004



Pluronic F-127 20% solution in DMSO



1ml



¥141.12



59005



Pluronic F-127, 10% in sterile H2O



30mL



¥505.68



59003



TPEN (Full name: tetrakis-(2-pyridylmethyl)ethylenediamine)



100mg



¥1117.2



FURA-2 and Furaptra
Fura-2 is a widely used UV-excitable fluorescent calcium indicator.1-5 Upon calcium binding, the fluorescent excitation maximum of the indicator undergoes a blue shift from 363 nm (Ca2+-free) to 335 nm (Ca2+-saturated), while the fluorescence emission maximum is relatively unchanged at ~510 nm. The indicator is typically excited at 340 nm and 380 nm respectively and the ratio of the fluorescent intensities corresponding to the two excitations is used in calculating the intracellular concentrations. Measurement of calcium concentration using this ratioing method avoids interference due to uneven dye distribution and photobleaching (Bright, G.R., et al, in Fluorescence Microscopy of Living Cells in Culture, Part B, (Methods in Cell Biology, Vol. 30), Academic Press (1989) p. 157). Fura-2 has been used in many cellular systems and applications, particularly in microscopic imaging.
Furaptra, which is also commonly called Mag-Fura-2, was originally developed as a fluorescent magnesium indicator. However, it is an excellent indicator for high calcium in the range of 1µM to 100 µM.6,7 Fura-2 and Furaptra share the same chromophore and as a result have similar spectral change in response to calcium concentration change.

Cat#


Product Name



Unit Size



Unit Price



50045



Bis-Fura-2, hexapotassium salt



1mg



¥1587.6



59100



Calcium Calibration Buffer Kit (zero and 10 mM CaEGTA, 2x50mL)



kit



¥1470



90082



DMSO, anhydrous (Dimethyl sulfoxide, or  methyl sulfoxide)



10mL



¥199.92



59002



EDC (EDAC; 1-(3-dimethylaminopropyl-3-ethylcarbodiimide, hydrochloride))



100mg



¥776.16



50033



Fura-2, AM ester



10100ug



¥1646.4



50033-1



Fura-2, AM ester



2050ug



¥1764



50034



Fura-2, AM ester



1mg



¥1352.4



50029



Fura-2, AM ester, 1mM in anhydrous DMSO



1ml



¥1622.88



50030



Fura-2, pentaammonium salt



1mg



¥1234.8



50031



Fura-2, pentapotassium salt



1mg



¥1234.8



50032



Fura-2, pentasodium salt



1mg



¥1234.8



50038



Furaptra, AM ester (also known as Mag-Fura-2, AM ester)



1mg



¥1999.2



50039



Furaptra, AM ester (also known as Mag-Fura-2, AM ester) (special packaging)



2050ug



¥2410.8



50037



Furaptra, AM ester (also known as Mag-Fura-2, AM ester) (special packaging)



10100ug



¥2175.6



50035



Furaptra, tetrapotassium salt (also known as Mag-Fura-2, tetrapotassium salt)



1mg



¥1528.8



50036



Furaptra, tetrasodium salt (also known as Mag-Fura-2, tetrasodium salt)



1mg



¥1528.8



59007



Ionomycin,calcium salt



1 mg



¥1552.32



59000



Pluronic F-127



1g



¥0



59004



Pluronic F-127 20% solution in DMSO



1ml



¥141.12



59005



Pluronic F-127, 10% in sterile H2O



30mL



¥505.68



59003



TPEN (Full name: tetrakis-(2-pyridylmethyl)ethylenediamine)



100mg



¥1117.2


Indo-1

Cat#


Product Name



Unit Size



Unit Price



59100



Calcium Calibration Buffer Kit (zero and 10 mM CaEGTA, 2x50mL)



kit



¥1470



90082



DMSO, anhydrous (Dimethyl sulfoxide, or methyl sulfoxide)



10mL



¥199.92



59002



EDC (EDAC; 1-(3-dimethylaminopropyl-3-ethylcarbodiimide, hydrochloride))



100mg



¥776.16



50044



Indo-1, AM ester



1mg



¥1352.4



50043-1



Indo-1, AM ester



2050ug



¥1764



50043



Indo-1, AM ester



10100ug



¥1646.4



50040



Indo-1, pentaammonium salt



1mg



¥1176



50041



Indo-1, pentapotassium salt



1mg



¥1176



50042



Indo-1, pentasodium salt



1mg



¥1176



59007



Ionomycin,calcium salt



1 mg



¥1552.32



59000



Pluronic F-127



1g



¥0



59004



Pluronic F-127 20% solution in DMSO



1ml



¥141.12



59005



Pluronic F-127, 10% in sterile H2O



30mL



¥505.68



59003



TPEN (Full name: tetrakis-(2-pyridylmethyl)ethylenediamine)



100mg



¥1117.2


Rhod-2

Cat#


Product Name



Unit Size



Unit Price



59100



Calcium Calibration Buffer Kit (zero and 10 mM CaEGTA, 2x50mL)



kit



¥1470



90082



DMSO, anhydrous (Dimethyl sulfoxide, or  methyl sulfoxide)



10mL



¥199.92



59002



EDC (EDAC; 1-(3-dimethylaminopropyl-3-ethylcarbodiimide, hydrochloride))



100mg



¥776.16



59007



Ionomycin,calcium salt



1 mg



¥1552.32



59000



Pluronic F-127



1g



¥0



59004



Pluronic F-127 20% solution in DMSO



1ml



¥141.12



59005



Pluronic F-127, 10% in sterile H2O



30mL



¥505.68



50024



Rhod-2, AM ester



1mg



¥1705.2



50023



Rhod-2, AM ester



10100ug



¥1975.68



50020



Rhod-2, triammonium salt



1mg



¥1528.8



50021



Rhod-2, tripotassium salt



1mg



¥1528.8



50022



Rhod-2, trisodium salt



1mg



¥1528.8



59003



TPEN (Full name: tetrakis-(2-pyridylmethyl)ethylenediamine)



100mg



¥1117.2


Other Related Products for Calcium Studies

Cat#


Product Name



Unit Size



Unit Price



59006



4-Bromo A-23187, free acid



1 mg



¥1293.6



59001



A-23187 Free Acid (calcimycin; or Calcium Ionophore III)



1mg



¥517.44



59100



Calcium Calibration Buffer Kit (zero and 10 mM CaEGTA, 2x50mL)



kit



¥1470



50050



DMNP-EDTA, tetrapotassium salt (Caged Calcium)



5 mg



¥1764



90082



DMSO, anhydrous (Dimethyl sulfoxide, or methyl sulfoxide)



10mL



¥199.92



59002



EDC (EDAC; 1-(3-dimethylaminopropyl-3-ethylcarbodiimide, hydrochloride))



100mg



¥776.16



59007



Ionomycin,calcium salt



1 mg



¥1552.32



59000



Pluronic F-127



1g



¥0



59004



Pluronic F-127 20% solution in DMSO



1ml



¥141.12



59005



Pluronic F-127, 10% in sterile H2O



30mL



¥505.68



50027



Probenecid, sodium salt *water soluble*



1077mg



¥1881.6



59003



TPEN (Full name: tetrakis-(2-pyridylmethyl)ethylenediamine)



100mg



¥1117.2


BAPTA Chelators

Cat#


Product Name



Unit Size



Unit Price



50004



5,5'-Dibromo BAPTA,tetrapotassium salt



100mg



¥1293.6



50005



5,5'-Difluoro BAPTA,AM ester



25mg



¥2175.6



50006



5,5'-Difluoro BAPTA,tetrapotassium salt



100mg



¥1764



50007



5,5'-Dimethyl BAPTA,AM ester



25mg



¥1705.2



50008



5,5'-Dimethyl BAPTA,tetrapotassium salt



100mg



¥823.2



50009



5-methyl-5'-nitro BAPTA,tetrapotassium salt



10mg



¥1940.4



50017



5-mononitro BAPTA,tetrapotassium salt



10mg



¥1940.4



50000



BAPTA,AM ester



25mg



¥1411.2



50000-1



BAPTA,AM ester



201mg



¥1764



50001



BAPTA,tetracesium salt



1g



¥2058



50002



BAPTA,tetrapotassium salt



1g



¥1352.4



50003



BAPTA,tetrasodium salt



1g



¥1352.4



59100



Calcium Calibration Buffer Kit (zero and 10 mM CaEGTA, 2x50mL)



kit



¥1470



90082



DMSO, anhydrous (Dimethyl sulfoxide, or  methyl sulfoxide)



10mL



¥199.92



59002



EDC (EDAC; 1-(3-dimethylaminopropyl-3-ethylcarbodiimide, hydrochloride))



100mg



¥776.16



59007



Ionomycin,calcium salt



1 mg



¥1552.32



59000



Pluronic F-127



1g



¥0



59004



Pluronic F-127 20% solution in DMSO



1ml



¥141.12



59005



Pluronic F-127, 10% in sterile H2O



30mL



¥505.68



59003



TPEN (Full name: tetrakis-(2-pyridylmethyl)ethylenediamine)



100mg



¥1117.2


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