TAEXO02kit_T TwistDx核酸检测试剂TwistAmp exoTAEXO02kit...
- 型号：TAEXO02kit_T
- 供应商：上海胜创生物科技有限公司
- 供应商报价：面议
- 标签：TwistDx核酸检测试剂TwistAmp exoTAEXO02kit_T,6560,上海胜创生物科技有限公司
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Basic Information
RPA1) Primers must be 30-35 bases2) Works best at constant temperature (37-39°C)3) Amplicons of 80-400bp are preferred4) TwistAmp? exo Probe required - see overleafSet-up (single-plex)11) Prepare reaction mix in 1.5ml tube: Primer A (10μM) 2.1 μl Primer B (10μM) 2.1 μl TwistAmp? exo Probe (10μM) 0.6 μl Rehydration Buffer 29.5 μl Template and dH2O 13.2 μl (Total Volume 47.5 μl) Vortex and spin briefly2) Add reaction mix to freeze-dried reaction. Pipette to mix.3) Add 2.5 μl of 280mM MgAc (supplied) and mix well to start reaction.4) Place strip in Twista? and start run: 37-39°C, 20 minutes.4b) For low template copy number, remove strip after 4 minutes, vortex & spin briefly, replace in Twista?.WARNING: RPA REACTIONS START AT ROOM TEMPERATURE AS SOON ASMAGNESIUM IS ADDED
