L6020F Enzymatics T7 DNA连接酶 T7 DNA Ligase ...
- 型号:L6020F
- 供应商:上海胜创生物科技有限公司
- 供应商报价:面议
- 标签:Enzymatics T7 DNA连接酶 T7 DNA Ligase L6020F,-1,上海胜创生物科技有限公司
PRODUCT SPECIFICATION*产品特性
Storage Temperature -25 to -15°C
Test Specification
Purity (SDS-PAGE) >99%
Specific Activity 3,000,000 U/mg
SS Exonuclease 30,000 U <1.0% released
DS Exonuclease 30,000 U <1.0% released
DS Endonuclease 30,000 U = No conversion
E.coli DNA Contamination 30,000 U <10 copies
* For a detailed summary of assay conditions and data, refer to the Quality Controls Analysis section.
NOTES
In the absence of high concentration crowding agents (eg 20-30% PEG 6-8000), the specific activity of T7 DNA Ligase is reduced by 1000-fold on blunt-ended fragments (1, 2).
当缺乏高浓度的拥挤试剂时(eg 20-30% PEG 6-8000),T7 DNA连接酶的特殊活性在平末端片段上将会被降低1000倍
T7 DNA Ligase is incapable of ligating single-stranded DNA fragments (1).
T7 DNA链接酶不能连接单链DNA片段
REFERENCES参考文献
Doherty, A. et al. J.Biol. Chem (1996) V.271, No.19, 11083-11089.
Dayal, T. Unpublished observations.
Limitations of Use适用范围
该产品被研制、生产加工及销售,仅用于试管内,不适用于人类和动物