IL2 白介素2(IL2)真核蛋白
- 品牌:美国eBioscience
- 型号:IL2
- 产地:中国/美国/德国
- 供应商:上海沪震生物科技有限公司
- 供应商报价:面议
- 标签:白介素2(IL2)真核蛋白,-1,上海沪震生物科技有限公司
HZA073Ra61 100ug Eukaryotic Interleukin 2 (IL2) Organism Species: Rattus norvegicus (Rat) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 11th Edition (Revised in May, 2016) [ PROPERTIES ] Source: Eukaryotic expression. Host: 293F cell Residues: Ala21~Gln155 Tags: N-terminal His Tag Homology: Human 66%, mouse 75% Subcellular Location: Secreted. Purity: >98% Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). Traits: Freeze-dried powder Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5%Trehalose and Proclin300. 白介素2(IL2)真核蛋白Original Concentration: 200ug/mL Predicted isoelectric point: 6.3 Predicted Molecular Mass: 17.1kDa Accurate Molecular Mass: 17/18kDa as determined by SDS-PAGE reducing conditions. Applications: SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.) Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows: 1. Splice variants: 白介素2(IL2)真核蛋白Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. [ USAGE ] Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex [ STORAGE AND STABILITY ] Storage: Avoid repeated freeze/thaw cycles. 白介素2(IL2)真核蛋白Store at 2-8 oC for one month. Aliquot and store at -80 oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 oC for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
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