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货号:AS13 2651- plant cell division protein ftsZ2抗体

产品信息

相关抗体:

货号:AS13 2651---植物细胞分裂蛋白(FtsZ2)抗体 

货号:AS09 413---植物细胞分裂蛋白FtsZ1/2抗体

货号:AS07 217---原核细胞分裂GTP酶抗体

货号:AS06 153---细胞分裂周期蛋白激酶2抗体

 

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plant cell division protein ftsZ2抗体(FtsZ2)介绍:

货   号:AS13 2651

中文名称:兔抗拟南芥植物细胞分裂蛋白(FtsZ2)抗体

英文名称:plant cell division protein ftsZ2抗体(FtsZ2)

应用:western blot (WB)

规格:50 µl

价格:3975元

 

plant cell division protein ftsZ2抗体(FtsZ2)简介:

PRODUCT INFORMATION
background 

FtsZ2 belongs to FtsZ family and binds to FtsZ1 and exhibits  GTPase activity. Component of a plastid division machinery.Alternative names: Plastid division protein FTSZ2-1AtFtsZ2-1, Plastid division protein FTSZ2-2, AtFtsZ2-2.

immunogen 

Recombinant part of Arabidopsis thalina FtsZ2 conserved in FtsZ2-1 O82533
At2g36250, FtsZ2-2 Q9LXJ0, AT3G52750

host rabbit
clonality polyclonal
purity serum
format lyophilized
quantity 50 µl
reconstitution For reconstitution add 50 µl of sterile water.
storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
tested applications western blot (WB)
related products AS07 217 | FtsZ | procaryotic cell division GTPase, cyanobacterial, rabbit antibody
AS09 413 | anti-FtsZ1 and 2 | plant cell division protein ftsZ1 and ftsZ2 , rabbit antibody 
AS10 715 | anti-FtsZ procaryotic cell division GTPase (bacterial), rabbit antibody
 
additional information antibody available in 3 various pack sizes: 50, 100 and 150 µl - please, inquire.
APPLICATION INFORMATION
recommended dilution 1: 2000 with enhanced ECL (WB)
expected | apparent MW 50.7 kDa
confirmed reactivity Arabidopsis thaliana, Hordeum vulgare
predicted reactivity Glycine max, Jatropha manihot, Ricinus communis
not reactive in no confirmed exceptions from predicted reactivity known in the moment
additional information to be added when available
selected references to be added when available, antibody released in June 2013.


application example

2 µg of recombinant FtsZ2 fragment(~10 kDa) (1) and total protein extracted with PEB (AS08 300) from leaf tissueArabidopsis thaliana (2) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with FtsZ2 antibody (AS13 2651, 1:2000, 1h) and secondary anti-rabbit (1:25 000, 1 h) antibody (HRP conjugated, secondary antibody AS09 602) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. The signal was developed for 5 min with ECL according to the manufacturer’s instructions. Exposure time was 2 min.

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