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063665 美国戴安Dionex ProPac HIC Protein and P...

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美国戴安Dionex ProPac HIC Protein and Peptide Column

Hydrophobic Interaction Chromatography Columns
for the Separation of Large Biomolecules

Hydrophobic Interaction Chromatography separates biomolecules in a decreasing salt gradient, based on differences in surface hydrophobicity. This method preserves the biological activity of proteins. The HIC separation mechanism is complementary to those of ion-exchange and gel filtration chromatography.

  • Based on 5 ?m ultrahigh purity spherical silica gel particles, with 300 ? pores
  • Proteins separated under non-denaturing conditions
  • High protein loading capacity for protein purification applications
  • Proprietary multidentate silica bonding for enhanced hydrolytic stability
  • High-resolution HPLC separation of proteins and peptides
  • Wide range of applications

The ProPac® HIC-10 column is a high-resolution, high-capacity, silica-based HIC column that provides greater hydrolytic stability under the highly aqueous conditions used in HIC. Examples include the separation of bovine serum proteins, snake venom proteins, enzymes, human skeletal muscle protein (HSMP), monoclonal antibodies, pancreatin, and thrombin and peptide applications including tryptic digests of proteins.

 

ProPac HIC Columns  Specifications
Protein Loading Capacity340 mg Lysozyme per 7.8 x 75 mm column
Starting MaterialUltrapure Spherical Silica
Particle Size5 ?m
Pressure Limit4,000 psi
Column ConstructionStainless Steel
PhaseHIC
Average Pore Diameter300 ?
EndcappedNo
Phase Compatibility2 M Ammonium sulfate/ phosphate salts, organic solvents for cleanup
pH rangepH 2.5 – 7.5
Temperature Limit
< 40 °C

Analytical Columns
ProPac HIC-10 Column (2.1 x 100 mm)063653
ProPac HIC-10 Column (4.6 x 100 mm)063655
ProPac HIC-10 (4.6 x 250mm)074197
ProPac HIC-10 Column (7.8 x 75 mm)063665
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