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冷冻切片质量提升系统(切片和染色)

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冷冻切片质量提升系统(切片和染色)

CryoJane™ Tape-Transfer System,可切取 2 micros 超薄冷冻切片,提升切片品质,避免破片、组织变形,可以解决fatty tissue & undecal cified bone 的切片问题,适用于 LCM 使用者。

整个系统含以下内容物(见图示):

  1. Flash-Pad Mechanism
  2. Electronics Control Unit
  3. Power Cord
  4. Door Channel
  5. Storage Shelf
  6. Hand Roller
  7. 125 Adhesive- Coated Slides
  8. Roll of 400 Tape Windows
  9. 2 Knife Facet Wipers
  10. Spare Fuse and 5 Block Holders (optional)

冷冻切片质量提升系统(切片和染色)订购信息:

货号

产品名称

规格

62800-20

CryoJane™ Tape-Transfer System冷冻切片质量提升系统

220Volt

 

使用步骤如下:

  1. 将TAPE WINDOWS (组织贴片带) 贴在蜡块表面
  2. 用锋利的切片刀切出适当厚度的frozen section
  3. 将整片粘有组织的 TAPE WINDOWS (组织贴片) 移到Adhensive coated slides
  4. 利用hand roller 将组织贴片更加平贴在slides上
  5. 将slides 移到Flash-Pad (UV 灯箱)中照射数秒
  6. UV会将slides上的coating转换成可固定住组织的solvent-resistant plastic
  7. 移除(组织贴片带)
  8. 可以继续后续的fixation & staing步骤

CryoJane™小附件简介:

货号

产品名称

产品描述

规格

62800-40

Block-Holder

Deep-waffled, large face block-holder securely anchors frozen embedding medium or tissue.

5/

62800-44

Microtome Chuck Adapter

Adapts to the microtome chuck for the use of Block-Holders above. (Please specify cryostat model when ordering.)

1

62800-46

Hand Roller

Required in the CryoJane™ process for laminating Tape Window to block-face

1

62800-48

Storage Shelf

Used for storing Adhesive Coated Slides and Tape Window inside the cryostat.

1

62800-50

Door Channel

Please specify model cryostat and whether Cryo-Vac-Away is installed.

1

62800-53

Fuses-1-Amp

SLO-BLOW ceramic core wound. Used in the CryoJane™ ECU

5/

62800-X

Adhesive Slides

Glass slide with adhesive coating, when polymerized, bonds the still-frozen section from the tape to the slide. Avoid thawing the section. ½ x coating slides for mild to normal tissues.

125/

62800-1X

Adhesive Slides

Glass slide with adhesive coating, when polymerized, bonds the still-frozen section from the tape to the slide. Avoid thawing the section. 1x coating slides for mild to normal tissues.

125/

62800-4X

Adhesive Slides

Glass slide with adhesive coating, when polymerized, bonds the still-frozen section from the tape to the slide. Avoid thawing the section. 4x coating slides for use with hard un-calcified bone) or fatty tissues.

100/

62800-72

Tape Windows

Applied to the block-face with the Hand Roller to capture the frozen section as it is being cut. Eliminate the need for a brush or anti-roll device.

400/

62800-74

Protective Oil

Used for coating frozen section blocks to prevent dehydration during long-term storage.

4OZ

62803-30

Protective Oil Accessories Kit

Temperature controlled Oil Bath, 4 oz Bottle of Protective Oil, and Oil Brush. Please note that installation of the Protective Oil Bath Accessory requires that the CryoJane™ system be returned to Electron Microscopy Sciences.

1

62800-76

Aqueous Buffer Salt Mix

Used in the preparation of the aqueous fixative, which preserves morphological detail in frozen sections. 2 vials per package, (each vial makes 180 ml of buffer).

2/

62800-78

Knife Facet Wipers

Effectively cleans both facets of knife-edge without dulling the blade. 10 wipers/pack

10/

 

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