11811023 G418 Sulfate（Geneticin）

现货供应Gibco公司G418产品

产品介绍：

G418（G-418即G-418 sulfate，也称Geneticin，分子式为C20H40N4O10.2H2SO4，分子量为692.71 g/mole）是一种氨基糖类抗生素，其结构与新霉素、庆大霉素、卡那霉素相似，它通过影响80S核糖体功能而阻断蛋白质合成,对原核和真核等细胞都有毒性 ,包括细菌、酵母、植物和哺乳动物细胞 ,也包括原生动物和蠕虫。是稳定转染Z常用的选择试剂。当neo基因被整合进真核细胞基因组合适的地方后 ,则能启动neo基因编码的序列转录为mRNA,从而获得抗性产物氨基糖苷磷酸转移酶的GX表达 ,使细胞获得抗性而能在含有Ｇ418的选择性培养基中生长。Ｇ418的这一选择特性 ,已在基因转移、基因敲除、抗性筛选以及转基因动物等方面得以广泛应用。
        G418和筛选
        筛选之前由于每种细胞对G418的敏感性不同，而且不同的厂家生产的相同浓度的G418的活性不尽相同，所以在筛选之前，一定要确定G418的Z佳筛选浓度。具体如下：将细胞稀释到1000个细胞/mL，在100ug/mL~1mg/mL的G418浓度范围内进行筛选，选择出在10~14天内使细胞全部死亡的Z低G418浓度来进行下一步的筛选试验。
        由于每种细胞对G418的敏感性不同，一般变动在100ug/ml～1000ug/ml范围。而且不同的厂家生产的相同浓度的G418的活性不尽相同，所以在筛选之前，一定要确定你的细胞对这一批G418的Z佳筛选浓度。尽管如此，特性明确的细胞系G418的Z佳用量还是稳定的。《分子克隆》给出了几个常用细胞系所需G418的Z佳用量：

订购信息：

我公司提供的G418来自Gibco公司，技术参数如下：
Shipping Conditions（运输条件）
GENETICIN is shipped at ambient temperature.
Storage Conditions（储存环境）
GENETICIN should be stored at room temperature, 15 to 30°C. GENETICIN is soluble in water and should be stored at 2 to 8°C as a solution.
Background
GENETICIN is an aminoglycoside related to Gentamicin and is used as a selective agent in molecular genetics experiments. It is toxic to both prokaryotic and eukaryotic cells. The resistance genes are dominant and are located on both Transposons Tn601 （903） and Tn5. The gene on Tn5 is referred to as a aminoglycoside phosphotransferase 3' （I） or APH（3'）I.The genes are bacterial in derivation but they can be expressed in eukaryotic cells. Efficient expression is achieved when the neo DNA sequence is linked to eukaryotic DNA sequences that permit transcription and processing of the Neo coding sequence into mRNA. Introduction of either of these genes into cells can confer resistance to GENETICIN, enabling the cells to grow in media containing GENETICIN. 
Description
GENETICIN is also known as Antibiotic G-418 Sulfate. GENETICIN is supplied
as a white powder. The amount of GENETICIN required to be present in culture media to select for resistant cells varies with a number of factors including cell type. Good laboratory practice requires that the optimal concentration of GENETICIN required to maintain and select cells must be determined for each set of growth conditions. Invitrogen recommends that whenever experimental conditions are altered （including use of GENETICIN from a different lot）, the optimal concentration of the product be re-evaluated.
Quality Control
Absorbance - Visible range: At a concentration of 100 mg/mL in water, GENETICIN gives a clear colorless solution. The absorbance of this solution at 400 nm is less than 0.075 O.D.
Absorbance - Ultraviolet range: At a concentration of 1 mg/mL in water, the absorbance at 280 nm is less than 0.015 O.D.