In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
HPAF-II is a human pancreatic adenocarcinoma cell line derived from peritoneal ascitic fluid of a 44 year old Caucasian male with primary pancreatic adenocarcinoma and metastases to the liver, diaphragm and lymph nodes. HPAF II is a spontaneous variant with unlimited replicative capability which proliferated from a static culture of HPAF-I cells.The cells express Muc 1 and Muc 4 mucin genes, and secrete high levels of Muc 1 mucin. They are pleomorphic and probably undergo some spontaneous differentiation in culture.
Propagation:
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing:
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Preservation:
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References:
21871: . Monoclonal antibodies in cancer. Clifton, NJ: Humana Press; 1985.
21882: . Monoclonal antibodies and cancer therapy. New York: Liss; 1985.
21891: . Immunity to cancer II. New York: Liss; 1989.
22431: Metzgar RS, et al. Antigens of human pancreatic adenocarcinoma cells defined by murine monoclonal antibodies. Cancer Res. 42: 601-608, 1982. PubMed: 7034925
22757: Kim YW, et al. Characterization of clones of a human pancreatic adenocarcinoma cell line representing different stages of differentiation. Pancreas 4: 353-362, 1989. PubMed: 2734279
22758: Mullins TD, et al. Ultrastructural differentiation of sodium butyrate-treated human pancreatic adenocarcinoma cell lines. Pancreas 6: 578-587, 1991. PubMed: 1946315
23087: Worlock AJ, et al. Radiolocalization of human pancreatic tumors in athymic mice by monoclonal antibody DU-PAN 1. Cancer Res. 50: 7246-7251, 1990. PubMed: 2224857
ATCC® Number: CRL-1997™ Price: $329.00 Designations: HPAF-II Depositors: RS Metzgar Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: adherent Organism: Homo sapiens (human) Morphology: epithelial Source: Organ: pancreas Disease: adenocarcinoma Cellular Products: mucin Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Tumorigenic: Yes Antigen expression: Blood Type A; Rh-; HLA A1, A10 (W34), B8, W22, Cw3 DNA Profile (STR): Amelogenin: X CSF1PO: 10,11 D13S317: 12 D16S539: 11,13 D5S818: 11,13 D7S820: 10,13 F13A01: 5,17 F13B: 8,10 FESFPS: 11,12 LPL: 10 THO1: 9 TPOX: 8 vWA: 17 Age: 44 years Gender: male Ethnicity: Caucasian Comments: HPAF-II is a human pancreatic adenocarcinoma cell line derived from peritoneal ascitic fluid of a 44 year old Caucasian male with primary pancreatic adenocarcinoma and metastases to the liver, diaphragm and lymph nodes. HPAF II is a spontaneous variant with unlimited replicative capability which proliferated from a static culture of HPAF-I cells.The cells express Muc 1 and Muc 4 mucin genes, and secrete high levels of Muc 1 mucin. They are pleomorphic and probably undergo some spontaneous differentiation in culture. Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Subculturing: Protocol: 1.Remove and discard culture medium. 2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. 3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 5.Add appropriate aliquots of the cell suspension to new culture vessels. 6.Incubate cultures at 37°C. 上海复祥生物提供 ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供 参考文献和优培养条件,网站上有细胞照片,联系邮箱xiangfbio@163.com.详情请登陆 http://www.xiangbio.com/欢迎各位老师来电咨询! iec-6 大鼠小肠隐窝上皮细胞 马-达二氏犬肾细胞系 MDCK (NBL-2) CRL-34 鸭子胚胎成纤维细胞 Duck embryo CCL-141 小鼠胚胎成纤维细胞 3T3-L1 CCL-173 人结肠直肠腺癌 COLO 320DM CCL-220 人结肠直肠腺癌 NCI-H508 CRL-253 人结肠直肠腺癌 COLO 320HSR CCL-220.1 人B淋巴细胞 Ramos（RA1） CRL-1596 人淋巴母细胞 T2(174×CEM.T2) CRL-1992 大鼠心肌细胞 H9C2(2-1) CRL-1446 CRL-1997 HPAF-II 人癌细胞 猪鼻甲黏膜成纤维细胞 PT-K75 CRL-2528 小鼠癌细胞 LTPA CRL-2389 大鼠癌细胞 DSL-6A/C1 CRL-2132 小鼠瘤B淋巴细胞 SP2/MIL-6 CRL-2016 大鼠脑间质细胞 DI TNC1 CRL-2005 小鼠肌原细胞 C2C12 CRL-1772 鹌鹑纤维肉瘤细胞 QT6 CRL-1708 大鼠回肠细胞 IEC-18 CRL-1589 非洲绿猴肾细胞 VERO C1008 [Vero 76, clone E6, Vero E6] CRL-1586 人成纤维细胞 Hs 738.St/Int CRL-7869 人正常前列腺上皮细胞 WPE-int CRL-2888 人正常前列腺成肌纤维细胞 WPMY-1 CRL-2854 人导管腺癌细胞 PL45 CRL-2558 人癌细胞 Panc 05.04 CRL-2557 人癌细胞 Panc 04.03 CRL-2555 人癌细胞 Panc 02.03 CRL-2553 人癌细胞 Panc 08.13 CRL-2551 人癌细胞 Panc 03.27 CRL-2549 人癌细胞 Panc 10.05 CRL-2547 人肝癌细胞 SNU-398 CRL-2233 人成神经瘤细胞- SK-N-DZ CRL-2149 人癌细胞 HPAC CRL-2119 人癌细胞 HPAF-II CRL-1997 人黑色素瘤细胞 A375.S2 CRL-1872 人脐静脉内皮细胞 HUV-EC-C CRL-1730 人黑素瘤细胞 WM-266-4 CRL-1676 人膀胱癌细胞 HT-1376 CRL-1472 人正常前列腺上皮细胞 RWPE-2 CRL-11610 人正常前列腺上皮细胞 RWPE-1 CRL-11609 鸡胚胎成纤维细胞 UMNSAH/DF-1 CRL-12203 人乳腺癌细胞 MDA-MB-231 HTB-26 CRL-1997 HPAF-II 人癌细胞 小鼠巨噬细胞 RAW 264.7 TIB-71 人横纹肌肉瘤细胞 A-204 HTB-82 人癌细胞 Capan-2 HTB-80 人癌细胞 Capan-1 HTB-79 人乳腺导管癌细胞 MDA-MB-435S HTB-129 人胃癌细胞 Hs 746T HTB-135 人肺腺癌细胞 NCI-H441 [H441] HTB-174 人瘤细胞 U266 TIB-196 人结肠癌细胞 NCI-H716 [H716] CCL-251 人多形性恶性胶质瘤 T98G [T98-G] CRL-1690 人神经母细胞瘤细胞 BE(2)-C CRL-2268 人肾透明细胞癌细胞 Caki-2 HTB-47 人皮肤成纤维细胞 Hs 865.Sk CRL-7601 CA46 人淋巴瘤细胞系 CRL-5826 NCI-H226 人肺鳞癌细胞系 Tca-8113 人舌鳞癌细胞 MA-891 小鼠乳腺癌高转移细胞 U14 小鼠宫颈癌细胞 CRL-9518 L5178Y 小鼠淋巴瘤细胞 HTB-112 HEC-1-A 人子宫内膜腺癌细胞 HTB-113 HEC-1-B 人子宫内膜腺癌细胞 AN3 CA 人子宫内膜腺癌细胞 ROS1728 大鼠成骨细胞 CRL-6323 B16F1 小鼠黑色素瘤细胞 HTB-58 SK-MES-1 人肺鳞癌细胞 CCL-127 IMR-32 人神经母细胞瘤细胞 HTB-79 Capan-2 人癌细胞 BALL-1 人B淋巴细胞急性白血病细胞 CRL-1997 HPAF-II 人癌细胞 CRL-2922 Eayh926 人脐静脉细胞融合细胞 WB-F344 大鼠肝干细胞 CRL-1715 TM4 正常小鼠睾丸细胞 CRL-2539 4T1 小鼠乳腺癌细胞 CRL-1442 BRL-3A 大鼠肝细胞 BRL 大鼠肝细胞 RSC96大鼠雪旺细胞 Isk 人子宫内膜癌细胞 HTB-113 hec-1-b 人子宫内膜腺癌细胞 PLA-802?人恶性横纹肌肉瘤 Pt-K2 袋鼠肾细胞 BS-C-1 非洲绿猴肾细胞 A498人肾癌细胞 3AO人卵巢癌细胞 iec-6 大鼠小肠隐窝上皮细胞 u87MG 人脑星形胶质母细胞瘤 Renca 小鼠肾癌细胞 nci-h929 瘤细胞 P388小鼠白血病细胞 CRL-1997 HPAF-II 人癌细胞 opm-2 瘤细胞 MDA-MB-435 人乳腺癌高转移细胞 KU812 人外周血嗜碱性白细胞 NIH:OVCAR-3 人卵巢癌细胞 SW 1990人癌细胞系 S180人结肠腺癌细胞 HuTu-80十二指肠腺癌 RL95-2 人子宫内腺癌细胞 NCI-H446 人小细胞肺癌细胞 MSTO-211H 人肺癌细胞 H4-Ⅱe 大鼠肝癌细胞系 HLF 人肺成纤维样细胞 LS 180人结肠腺癌细胞 HuTu-80十一指肠腺癌 ST猪睾丸细胞系 REH人急性非B非T淋巴细胞白血病 NCI-H520 人肺鳞癌细胞 RKO 人结肠腺癌细胞 293A 人胚肾细胞 RK-13 兔肾细胞系
