14932S Acetyl-Histone H3 （Lys23） （D6Y7M） R...
- 型号：14932S
- 产地：美国
- 供应商：上海睿铂赛生物科技有限公司
- 供应商报价：面议
- 标签：Acetyl-Histone H3 （Lys23） （D6Y7M） Rabbit mAb,3900,上海睿铂赛生物科技有限公司
产品库
Species predicted to react based on sequence homology: Hamster, Zebrafish
Specificity / Sensitivity
Acetyl-Histone H3 (Lys23) (D6Y7M) Rabbit mAb recognizes endogenous levels of histone H3 protein only when acetylated at Lys23. This antibody does not cross-react with histone H3 acetyl-lysine 9, 14, 18, 27, or 56.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding acetyl-Lys23 of human histone H3 protein.
Western Blotting
Western blot analysis of lysates from HeLa and C6 cells, untreated (-) or treated with TSA #9950 (1 μM, 18 hr; +), using Acetyl-Histone H3 (Lys23) (D6Y7M) Rabbit mAb (upper) and Histone H3 (D1H2) XP®Rabbit mAb #4499 (lower).
Western Blotting
Acetyl-Histone H3 (Lys23) (D6Y7M) Rabbit mAb antibody specificity was determined by western blotting. HeLa cell lysates, untreated (-) or treated with TSA #9950 (1 μM, 18 hr; +), were probed with Acetyl-Histone H3 (Lys23) (D6Y7M) Rabbit mAb (panel A) or Acetyl-Histone H3 (Lys23) (D6Y7M) Rabbit mAb pre-adsorbed with 1.5 μM of various competitor peptides (panels B-K). As shown, only the acetyl-histone H3 (Lys23) peptide competed away binding of the antibody
