产品库
登录
首页 找仪器 社区 百科 新品 供应商 品牌 应用 资料 仪企号 展会 标准 求购 招中标

重组人血管内皮生长因子受体-1蛋白D1-3/FLT1 D3 Human

产品信息
重组人血管内皮生长因子受体-1蛋白D1-3/FLT1 D3 Human
产品编号：PKA-234
产品规格：2μg/10μg/100μg
Catalogue NumberPKA-234
Synonyms FLT-1, FLT1, Tyrosine-protein kinase receptor FLT, Flt-1, Tyrosine-protein kinase FRT, Fms-like tyrosine kinase 1, VEGFR-1.
Introduction Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVE supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA. The biological functions of sVEGFR-1 still are not clear, but it seems to be an endogenous regulator of angiogenesis, binding VEGF with the same affinity as the full-length receptor.
Description Soluble FLT1 D1-3 Human Recombinant produced in baculovirus is monomeric, glycosylated, polypeptide containing 352 amino acids and having a molecular mass of 45 kDa. The soluble receptor protein contains only the first 3 extracellular domains, which contain all the information necessary for binding of VEGF.

The FLT1 is purified by proprietary chromatographic techniques.
Source Insect Cells.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation FLT1 D1-3 was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.
Solubility It is recommended to reconstitute the lyophilized FLT1 D3 in sterile water not less than 100 μg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized FLT-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FLT1 should be stored at 4°C between 2-7 days and for future use below

-18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.
Purity Greater than 90.0% as determined by(a)Analysis by RP-HPLC.

(b)Analysis by SDS-PAGE.
Biological Activity The activity of FLT1D1-3 was determined by its ability to abolish the binding of iodinated VEGF to solid surfaces or cell surfaces receptors, and in Far-Western and cross-linking experiments with iodinated VEGF.
Amino Acid Sequence

Signal Peptide:

MVSYWDTGVLLCALLSCLLLTGSSSG.

Soluble sFlt.1(D3):

SKLKDPELSLKGTQHIMQAGQTLHLQCRGEAAHKWSLPEMVSKESERLSITKS

ACGRNGKQFCSTLTLNTAQANHTGFYSCKYLAVPTSKKKETESAIYIFISDTGR

PFVEMYSEIPEIIHMTEGRELVIPCRVTSPNITVTLKKFPLDTLIPDGKRIIWDSRK

GFIISNATYKEIGLLTCEATVNGHLYKTNYLTHRQIIDVQISTPRPVKLLRG

HTLVLNCTATTPLNTRVQMTWSYPDEKNKRASVRRRIDQSNSHANIFYSVLTID

KMQNKDKGLYTCRVRSGPSFKSVNTSVHIYDKAFITVKH.


Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
信息声明：本产品供应信息由仪器网为您整合，供应商为（厦门慧嘉生物科技有限公司），内容包括 （ 重组人血管内皮生长因子受体-1蛋白D1-3/FLT1 D3 Human）的品牌、型号、技术参数、详细介绍等；如果您想了解更多关于 （ 重组人血管内皮生长因子受体-1蛋白D1-3/FLT1 D3 Human）的信息，请直接联系供应商，给供应商留言！
供应商产品推荐
    您可能感兴趣的产品