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TIMP1 Human, HEK/ 重组人金属蛋白酶1组织YZ物, HEK

产品信息
TIMP1 Human, HEK/ 重组人金属蛋白酶1组织YZ物, HEK
产品编号：ENZ-508
产品规格：2μg/10μg/0.1mg
Synonyms Metalloproteinase inhibitor 1, Tissue inhibitor of metalloproteinases, TIMP-1, Erythroid-potentiating activity, EPA, Fibroblast collagenase inhibitor, Collagenase inhibitor, TIMP1, CLGI, TIMP, EPO, HCI, FLJ90373.
Introduction TIMP1 is a member of the TIMP family. TIMP1 is an inducible glycoprotein produced by various cell types. The TIMP1 glycoprotein is a natural inhibitor of the matrix metalloproteinases, which a group of peptidases involved in degradation of the extracellular matrix. TIMP1 binds in a reversible mode to MMPs, with regions in the N-terminal domain binding to the MMP substrate-binding site. On top of its inhibitory function against most of the known MMPs, TIMP1 is able to promote cell proliferation in a broad range of cell types, and may also have an anti-apoptotic role. Furthermore, TIMP1 has erthyroid-potentiating activity via translocation to the nucleus and also inhibits apoptosis in B-cells. The TIMP1 gene is situated within intron 6 of the synapsin I gene and is transcribed in the opposite direction. TIMP1 activity is dependent on the existence of disulfide bonds. TIMP1 transcription is extremely inducible in reaction to many cytokines and hormones. Increased TIMP1 levels are connecte
Description TIMP1 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain containing 184 amino acids with a molecular weight of 20.7 kDa though on SDS-PAGE migrates at about 28kDa due to the glycosilation. The TIMP1 is purified by proprietary chromatographic techniques.
Source HEK293-F Cells.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation The TIMP1 protein was lyophilized from a 0.2μm filtered solution containing no additives or preservatives.
Solubility It is recommended to reconstitute the lyophilized TIMP1 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized TIMP1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TIMP1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA.
Biological Activity IC50 = 0.4 to 1.2 nM, determined by the concentration dependent inhibition of human MMP-2 hydrolysis of a peptide substrate (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-(3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl)-Ala-Arg-NH2, under conditions in which recombinant pro-MMP-2 is activated, and the peptide substrate concentration is 5μM.
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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