产品信息
人扁桃体上皮细胞
上海义森生物科技有限公司
Description
Tonsil epithelium is a heterogeneous lymphoid organ containing two different types of actively differentiating epithelia, the lining stratified squamous epithelium and the reticulated crypt, or lymphoepithelium. The tonsil crypts represent a specialized compartment, important in the immunological functions of the tonsil because of the immediate proximity of the epithelial and lymphoid tissues [1]. In Situ staining of tonsil epithelium suggests that expression of the simple epithelial keratins K8, K18 and K19 is located to the crypt epithelial cells [2]. Cell culture of tonsil epithelium produces heterogeneous squamous epithelial culture in which differentiation appears to be ongoing and keratin expression patterns are consistent with those found in tonsil epithelial cells in situ [3].
HTEpiC from ScienCell Research Laboratories are isolated from human normal tonsil tissue. HTEpiC are cryopreserved at passage one culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HTEpiC are characterized by immunofluorescent method with cytokeratine antibodies. HTEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HTEpiC are guaranteed to further culture at the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Tonsil Epithelial Cell Medium (TEpiCM, Cat No 2561) for expanding HTEpiC in vitro.
Product Use
HTEpiC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Perry, M. E. (1994) The specialized structure of crypt epithelium in the human palatine tonsil and its functional significance. J. Anat. 185:111–127.
[2] Clark, M. A., C. Wilson, A. Sama, J. A. Wilson, and B. H. Hirst. (2000) Differential cytokeratin and glycoconjugate expression by the surface and crypt epithelia of human palatine tonsils. Histochem. Cell Biol. 114:311–321.
[3] Dirk M. Pegtel, Jaap Middeldorp, and David A. Thorley-Lawson (2004) Epstein-Barr virus infection in ex vivo tonsil epithelial cell cultures of asymptomatic carriers. J. Virology. 78(22):12613–12624.
