RPK09 Proteinase K,Recombinant Proteinase...
- 品牌：上海雅心生物技术有限公司
- 型号：RPK09
- 产地：NO 466,Yindu Road,Xuhui,Shanghai
- 供应商：上海雅心生物技术有限公司
- 供应商报价：190
- 标签：Proteinase K,Recombinant Proteinase K,190,上海雅心生物技术有限公司
产品库
Recombinant Proteinase K
For research use only
Catalog Number:RPK09
Cas:39450-01-6
EC:3.4.21.14
Alternate names:Protease K, Endopeptidase K, Tritirachium alkaline proteinas
DESCRIPTION
A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF).
ADVANTAGES
Format | Lyophilized |
M.W | 29.3kD |
Purity | 99% |
Activity | 30 units/mg |
DNAses and RNAses | not detected |
UNIT DEFINITION
One unit is defined as the amount of enzyme that will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 mol tyrosine per min at 37°C, pH 7.5(color by Folin-Ciocalteu reagent).
RECOMMEND USAGE
Prepare recombinant proteinase K with 20mM Tris-HCl (pH 7.4~8.0),1mM CaCl2 ,50% glycerol.The optimum pH is 8.0.
STORAGE INSTRUCTIONS
Store lyophilized at -20°C. Aliquot the product after reconstitution to avoid repeated freezing thawing cycles.
HANDLING
Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.
