RTS04 Recombinant Trypsin Solution

Animal Origin Free Recombinant Trypsin Solution, ready-to-use

          Animal Origin Free, No Virus Contaminant

DESCRIPTIONS

Recombinant trypsin solution, is an animal component-free trypsin solution optimized for cell

dissociation. It is formulated with a recombinant human trypsin expressed in E.Coli and refolded and purified with chromatography. The sequence of human recombinant trypsin is same as human pancreas trypsin 2.

FEATURES AND BENEFITS:

Animal component-free

Recombinant trypsin eliminates the risk of viruses, or other potential adventitious agents

Trypsin is recombinant trypsin

Recombinant trypsin owns a similar kinetics for cell detachment with native trypsin. Soybean trypsin inhibitor and other inhibitor work the same with recombinant human trypsin as they do with native trypsin.

High purity

Recombinant trypsin provides increased specificity and eliminates contaminating activities found in lower purity enzymes

Convenience

Recombinant trypsin solution is formulated at the optimal concentration to dissociate adherent cells

High safety

The solution, ready-to-use reagent eliminates the chance of activity loss in the process of transport and

storage, and eliminates the contamination opportunity as a sterile recombinant trypsin lyophilized powder.

USAGE METHOD：

Step 1. Withdraw 1 ml cool balance solution into EP tube containing trypsin powder.

Step 2. Dissolve trypsin powder thorough, and then remove all to

the vial containing balance solution, mixing. Best use it within 4hs.

NOTES:

Note 1. Dissociate adherent cells under room temperature. 37 ℃ is not recommended.

Note 2. If necessary, after dissolving immediay dispense into suitable containers, then stored under -20℃. When use, take it from -20℃ and dissolve in room temperature.

Figure 1. SDS-PAGE analysis on the recombinant trypsin

PRODUCTS INFORMATION

UNIT DEFINITION：One BAEE unit of trypsin activity will produce a Delta A253 of 0.001 per minute in a reaction volume of 3.0ml at pH7.6 at 25℃ （1cm light path）.

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