ab176112 Frataxin Human SimpleStep ELISA Kit

Product nameFrataxin Human SimpleStep ELISATM Kit See all Frataxin kitsDetection methodColorimetricPrecision Intra-assay Sample n Mean SD CV% HeLa 9 2.3% Inter-assay Sample n Mean SD CV% HeLa 3 6.3% Sample typeCell culture extracts, Tissue ExtractsAssay typeSandwich (quantitative)Sensitivity 2.1 pg/mlRange1.1 pg/ml - 800 pg/mlRecovery Sample specific recovery Sample type Average % Range Serum 92 86% - 98% Cell culture media 97 93% - 101% EDTA Plasma 89 88% - 92% Assay time1h 30m Assay durationOne step assaySpecies reactivityReacts with: Human Product overview Abcam’s Frataxin in vitro SimpleStep ELISA? (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Frataxin protein in Human cell culture and tissue extracts. The SimpleStep ELISA? employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm Notes Frataxin is a 17 kDa nuclear-encoded mitochondrial protein. In humans the gene is localized on chromosome 9 and is highly conserved during evolution. The gene is expressed in every cell, although in varying levels in different tissues and during development. The specific function of frataxin is still unknown, but it has been shown to play a role in iron metabolism. Studies have demonstrated that the deletion of the frataxin gene in yeast results in iron accumulation in mitochondria and loss of respiration. Recombinant human frataxin has been shown to bind iron in vitro, and increased mitochondrial iron levels have been observed in patients with Friedreich's Ataxia (FRDA). FRDA is an autosomal recessive, progressive degenerative disease characterized by neurodegeneration and cardiomyopathy it is the most common inherited ataxia. Tested applicationsSandwich ELISAmore details PlatformMicroplate Properties Storage instructionsStore at +4°C. Please refer to protocols. Components 1 x 96 tests 10X Frataxin Capture Antibody 2 x 300μl 10X Frataxin Detector Antibody 2 x 300μl 10X Wash Buffer PT 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 2 x 3ml Frataxin Human Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (12 x 8 wells) 1 unit Stop Solution 1 x 12ml TMB Substrate 1 x 12ml Research Areas Cell Biology Apoptosis Mitochondrial Signal Transduction Metabolism Mitochondrial Signal Transduction Protein Trafficking Chaperones Other Chaperones Signal Transduction Metabolism Vitamins / Minerals Cell Biology Other Antibodies Oxidative Stress Kits/ Lysates/ Other Kits ELISA Kits ELISA Kits Apoptosis marker and proteins ELISA kits Kits/ Lysates/ Other Kits ELISA Kits ELISA Kits Signal transduction proteins ELISA kits Metabolism Pathways and Processes Mitochondrial Metabolism Mitochondrial markers Metabolism Pathways and Processes Metabolism processes Apoptosis RelevanceFunction: Promotes the biosynthesis of heme and assembly and repair of iron-sulfur clusters by delivering Fe2+ to proteins involved in these pathways. May play a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe2+ to Fe3+; the oligomeric form but not the monomeric form has in vitro ferroxidase activity. May be able to store large amounts of iron in the form of a ferrihydrite mineral by oligomerization; however, the physiological relevance is unsure as reports are conflicting and the function has only been shown using heterologous overexpression systems. Modulates the RNA-binding activity of ACO1.Cellular localizationCytoplasmic and MitochondrialAlternative names CyaY FA FARR see allDatabase links Entrez Gene: 2395 Human Omim: 606829 Human SwissProt: Q16595 Human Applications Our Abpromise guarantee covers the use of ab176112 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application Abreviews Notes Sandwich ELISA Use at an assay dependent concentration.