重组人干扰素-α 1b （rHuIFN-alpha 1b）

Background:   At least 23 different variants of IFN-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.   Description :   Recombinant Human Interferon alpha-1b produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 166 amino acids and having a molecular mass of 19392 Dalton. The Interferon-alpha 1b gene was obtained from human leukocytes. The IFN-alpha-1b is purified by proprietary chromatographic techniques.   Physical Appearance:   Sterile Filtered White lyophilized （freeze-dried） powder.   Formulation:   Lyophilized from a （1mg/ml） solution in containing 20mg HSA and 7.5mg sodium chloride. Solubility:   It is recommended to reconstitute the lyophilized IFN alpha-1b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.   Stability:     Lyophilized IFN-alpha-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution IFN-alpha-1b should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles.   Purity:   Greater than 98.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.   Amino acid sequence:   The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Glu.   Dimers and aggregates:   Less than 1% as determined by silver-stained SDS-PAGE gel analysis.   Biological Activity:   ProSpec's Interferon-alpha 1b is fully biologically active when compared to WHO standard. The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 1 x 108 IU/ mg.   Endotoxin:   Less than 0.1 ng/µg （IEU/µg） of IFN-alpha 1b.   Protein content:   Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC, using a standard solution of IFN-alpha 1b as a Reference Standard.   Usage:   Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Latest Publications:   1. [Gene modification and high prokaryotic expression of porcine interferon alpha-1] Sheng Wu Gong Cheng Xue Bao 2004 Mar;20（2）:291-4 2. [Translational control of human interferon alpha 1 gene expression in E.coli] Zhongh ua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 2002 Dec;16（4）:315-8 3. Differential effects of angiostatin, endostatin and interferon-alpha（1） gene transfer on in vivo growth of human breast cancer cells. Gene Ther 2002 Jul;9（13）:867-78 4. High-yield expression and purification of human interferon alpha-1 in Pichia pastoris. Protein Expr Purif 2001 Aug;22（3）:381-7 5. Unusual effect of clusters of rare arginine （AGG） codons on the expression of human interferon alpha 1 gene in Escherichia coli. Int J Biochem Cell Biol 1997 Apr;29（4）:659-66 6. Extracellular matrix remodelling in a murine mammary adenocarcinoma transfected with the interferon-alpha 1 gene. J Pathol 1997 Jan;181（1）:116-23

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