R7876 Ribonucleic acid, transfer from whe...

Description

Unit  Definition      One unit will yield an absorbance of 1.0 at 260 nm in 1.0 mL of water    （1cm light path）.
Application      Crude transfer RNA （tRNA）, purified from wheat germ, contains many specific tRNA molecules that can be separated by different processes. Experiments for detailed investigation of structure and function using in vitro translation systems often require highly purified tRNA, which can be achieved by combinations of chromatographic separations and reversible biochemical modification of the tRNA.1 Wheat germ tRNA has been used for the purification of RNAse Z （tRNA 3′- processing endonucleases） from wheat germ. The purification included six steps with the most efficient one using an affinity column made of wheat germ tRNA, which the RNAse Z bound tightly.2

Properties
  type               Type V
  assay              15-19 unit/mg solid
  form               lyophilized powder
  storage condition  desiccated
  EQP level          Premium
  solubility         H2O: ~2 mg/mL, clear to hazy
  storage temp.      2-8°C

Safety
  Personal Protective Equipment     Eyeshields, Gloves, type N95 （US）, type P1 （EN143）   respirator filter
  Safety Statements                 22-24/25
  WGK Germany                        3