Lentivirus Titer Kit （Lentivirus-A...

QuickTiter™ Lentivirus Titer Kit （Lentivirus-Associated HIV p24 ELISA）

Free p24 protein may account for a substantial por-

tion of total p24 in lentiviral supernatant. The tradi-

tional p24 ELISA detects both virus-associated p24

and free p24 generated by 293T cells during transient

transfection. Our QuickTiter™ Lentivirus Titer Kit

minimizes the overestimation of p24 in lentivirus su-

pernatant. Our proprietary technology separates the

lentivirus-associated p24 from free p24 protein prior

to performing the ELISA. 

•   More Accurate: Exclusive technology minimizes

overestimation of virus titer

•   User-Friendly: Simple incubation followed by

ELISA

•   Fully Quantitative: p24 Standard included

1.8

p24 in supernatant

1.6

p24 in pellet

1.4

1.2

1

0.8

0.6

0.4

0.2

0

0

25

50

75

100

Assay Principle. Upon transfection of lentiviral packaging plas-

p24 （ng/mL）

Free p24 Does not Complex with ViraBind™ Reagents. Recom-

binant p24 was diluted in culture medium and treated with Vira-

Bind™ Lentivirus Reagents A and B found in the QuickTiter™

Lentivirus Titer Kit. The amount of p24 in the supernatant and the

pellet was measured according to the assay protocol.

mids into 293T cells, lentivirus particles are packaged with p24

protein. However, additional p24 protein not associated with virus is

present in viral supernatant. A traditional p24 ELISA will detect both

sources of p24 which overestimates viral titer. The QuickTiter™

Lentivirus Titer Kit uses proprietary technology to pull the virus out

of solution prior to quantitation, providing a much more accurate

measurement of viral titer.