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24-Well Wound Healing Assay

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CytoSelect™ 24-Well Wound Healing Assay


Wound healing assays are useful for studying tissue
matrix remodeling, regulation of cytoskeletal struc-
ture, and cell proliferation and migration rates of dif-
ferent cells and culture conditions. Traditional wound
healing assays are performed by making a scratch
across a confluent cell monolayer to create an open
gap, mimicking a “wound”. Such scratch assays,
however, lack a consistently defined wound area
and result in high inter-sample variation.
 
Our CytoSelect™ 24-Well Wound Healing Assay
provides a more consistent method to measure cell
migration across a “wound field” gap in vitro. Pro-
prietary treated inserts generate a consistently de-
fined 0.9mm gap between the cells. Cells can then
be treated and monitored for proliferation or migra-
tion across the wound field by imaging samples at
fixed time points or time-lapse microscopy.
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