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ABTS diammonium salt,30931-67-0

产品信息
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    YZ剂描述:

    产品名称:ABTS diammonium salt

    产品别名:见爱必信官网

    英文别名:ABTS diammonium salt

    靶点:Biochemical Assay Reagent

    CAS:30931-67-0

    纯度:98%

    外观:见爱必信官网

    保存方法:store at -20℃ for one year(Powder)

    描述:ABTS diammonium salt is a substrate for horseradish peroxidase (HRP) conjugate.

    溶解性:

    In Vitro: 

    H2O : ≥ 50 mg/mL (91.13 mM)

    * "≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    ConcentrationSolventMass1 mg5 mg10 mg
    1 mM1.8226 mL9.1128 mL18.2256 mL
    5 mM0.3645 mL1.8226 mL3.6451 mL
    10 mM0.1823 mL0.9113 mL1.8226 mL
    *请根据产品在不同溶剂中的溶解度,选择合适的溶剂配制储备液,并请注意储备液的保存方式和期限。

    体外研究:

    A micro-technique of enzyme-linked immunosorbent assay (ELISA) using ABTS, as a substrate for HRP conjugate is studied. In a comparative study among 4 substrates, namely; 5-aminosalicylic acid (S), O-phenylenediamine (OPD), O-tolidine (OT) and ABTS, for HRP in terms of sensitivity, ABTS is the most sensitive, stable and the best in visuality by its bluish-green color. ABTS is a typical peroxidase substrate. For purification and characterization peroxidase positive transformants are cultivated in large scale (XL) under conditions that yield active protein in the culture supernatant. After 160 h cultivation an activity of 55,000 U/L in relation to the substrate ABTS is achieved and the supernatant containing the peroxidase is harvested. With ABTS as substrate the peroxidase activity falls significantly when the H2O2 concentration rose above 0.125 mM, indicating that the enzyme is inhibited by H2O2. Maximum reaction rates depending upon substrate tested reache values between 31.2 and 125 µM.

    体内研究:

    产品信息订购:

      产品货号   产品名称   规格 价格 大包装及货期
      abs821919   ABTS diammonium salt   1g  696   立即咨询

    产品更多信息请进入爱必信网站咨询

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