Coelenterazine,55779-48-1

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YZ剂描述：

产品名称：Coelenterazine

产品别名：腔肠素

英文别名：Coelenterazine

靶点：Others

CAS：55779-48-1

纯度：98%

外观：yellow solid

保存方法：store at -20℃ for one year（Powder）； in DMSO or others solvent store at 2-4℃ for two weeks, at -20℃ for six months.

描述：

Coelenterazine is a luminescent enzyme substrate, used for monitoring reporter genes in BRET, ELISA and HTS techniques.

溶解性：DMSO ：84mg/mL(198.36mM)
Ethanol ：1mg/mL(2.36mM)

体外研究：

When monitoring various biological processes, coelenterazine (CTZ) can be catalyzed by luciferases, such as the sea pansy Renilla reniformis (Rluc) and the marine copepod Gaussia princeps (Gluc), which results in emission of blue light. In Gli36-Gluc- or Gli36-Rluc-expressing Gli36 cells, coelenterazine (8 μM/ml) shows a bioluminescence signal. In RIN-5F, 4T1, HCT 116, HEK 293, 293FT, HeLa and INS-1 cells, coelenterazine can be used to assay superoxide anion concentrations.

体内研究：In 4T1-implanted mouse, Coelenterazine detects cancer-associated superoxide anion and used to evaluate the role of superoxide anion in cancer cell biology and its role in pathology.

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