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SB 203580 hydrochloride,869185-85-3

产品信息

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    YZ剂描述：

    产品名称：SB 203580 hydrochloride

    产品别名：RWJ 64809 hydrochloride

    英文别名：RWJ 64809 hydrochloride

    靶点：p38 MAPK,PKB

    CAS：869185-85-3

    纯度：98%

    外观：Powder

    保存方法：store at -20℃ for one year（Powder）； in DMSO or others solvent store at 2-4℃ for two weeks, at -80℃ for six months.

    描述：

     SB 203580 hydrochloride is a p38 MAPK inhibitor with IC50 of 0.3-0.5 μM, also blocks PKB phosphorylation with IC50 of 3-5 μM.

    溶解性：Soluble in ethanol (~30 mg/ml), DMSO (~30 mg/ml), DMF (~30 mg/ml), 1:10 solution of ethanol:PBS(pH 7.2) (~0.1 mg/ml), and water (25 mM).

    体外研究：

    SB 203580 (preincubated with 0-30 μM for 1 h and cultured for 24 h in the presence of 20 ng/mL IL-2) prevents the IL-2-induced proliferation of primary human T cells, murine CT6 T cells, or BAF F7 B cells with an IC50 of 3-5 μM.
    SB203580 blocks PKB phosphorylation (IC50 3-5 μM). SB203580 inhibitsthe phosphorylation of Ser473 in a dose-dependent manner in both CT6 and activated human T cells and IL-2-responsive BA/F3 F7 B cells.

    Cell Proliferation Assay

    Cell Line:CT6, BA/F3 cell line F7, and PBMC/T cells
    Concentration:0-30 μM
    Incubation Time:Preincubated with 0-30 μM SB203580 for 1 h and cultured for 24 h in the presence of 20 ng/mL IL-2
    Result:Prevented the IL-2-induced proliferation of primary human T cells, murine CT6 T cells, or BAF F7 B cells with an IC50 of 3-5 μM.

    体内研究：SB 203580 decreases protein concentrations of IL-1β from 106.49±10.93 to 67.85±7.39 pg/mL and TNF-α from 462.54±50.16 to 252.71±44.03 pg/mL. Similarly, the protein levels of MMP-2 and MMP-9 are significantly lower in the SB 203580 than the EM group. After treatment with SB 203580, the protein levels of MMP-2 and MMP-9 decreases from 2.70±0.14 to 1.74±0.26 ng/mL and from 3.17±0.31 to 1.98±0.24 ng/mL, respectively. SB 203580 is evaluated in several models of cytokine inhibition and inflammatory disease. It is demonstrated clearly to be a potent inhibitor of inflammatory cytokine production in both mice and rats with IC50 values of 15 to 25 mg/kg.

    产品信息订购：

      产品货号   产品名称   规格 价格 大包装及货期
      abs812866   SB 203580 hydrochloride   10mg  500   立即咨询

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