Product Name CD3 epsilon monoclonal antibody
Catalog No PAab09892
Packing 100ul
Form liquid
Alternative Name CD3, CD3 epsilon, CD3E, CD3-epsilon, T3E, TCRE
Purification Protein A+G purification
Purity 95% as determined by SDS-PAGE
Host Rabbit
Isotype IgG
Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)
Background/ FUNCTION
CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a monoclonal antibody directed against the epsilon chain of human CD3 molecule.
Immunogen CD3e molecule, epsilon (CD3-TCR complex)
Specificity Human
Tested Application ELISA, WB, IHC
Recommended dilution WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
IHC use Immunohistochemistry of paraffin-embedded human spleen tissue slide using PAab09892( CD3E Antibody) at dilution of 1:100
WB use
Jurkat cells were subjected to SDS PAGE followed by western blot with PAab09892( CD3E Antibody) at dilution of 1:1000
Protein Information
Gene ID 916
Uniprot ID P07766
Calculated MW 85 kDa
Research Area Cancer, Immunology, Signal Transduction
