Recombinant Human Legumain/Asparaginyl Endopeptidase

公司供应的产品仅用于科研
产品名称：Recombinant Human Legumain/Asparaginyl Endopeptidase
Known as    Legumain; Asparaginyl Endopeptidase; Protease Cysteine 1; LGMN; PRSC1
Derived from    Human cells
Purity    Greater than 95% as determined by reducing SDS-PAGE.
Formulation    Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, 10% Glycerol, pH 8.0.
Shipping    The product is shipped on dry ice/ice packs.
Storage    Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months
Endotoxin    Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Background    Legumain is a lysosomal cysteine protease which is a member of the peptidase C13 family. Though it is found in many tissues, it is highly expressed in the kidney, heart, and placenta. Legumain has a strict specificity for hydrolysis of asparaginyl bonds and can also cleave aspartyl bonds slowly, especially under acidic conditions. Over-expression Legumain in tumors is significant for invasion and metastasis. In addition, Legumain may be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system and negative regulation of neuron apoptosis.
Recombinant Human Legumain/Asparaginyl Endopeptidase is produced by our Mammalian expression system and the target gene encoding Ile18-Tyr433 is expressed with a 6His tag at the C-terminus.
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