Citrate synthetase

【Molecular Wt.】52 kDa

【Description】Citrate synthase is found in nearly all cells capable of oxidative metabolism.The enzyme citrate synthase exists in nearly all living cells and stands as a pace-making enzyme in the first step of the citric acid cycle (or Krebs cycle). Citrate synthase is localized within eukaryotic cells in the mitochondrial matrix, but is encoded by nuclear DNA rather than mitochondrial. It is synthesized using cytoplasmic ribosomes, then transported into the mitochondrial matrix. Citrate synthase is commonly used as a quantitative enzyme marker for the presence of intact mitochondria.

【Species Reactivity】Human;Mouse;Rat

【Tested applications】WB;IHC;FC

【Antibody type】Recombinant Rabbit monoclonal Antibody

【Immunogen】Recombinant protein

【Positive control】Mouse brain tissue lysate, rat kidney tissue lysate, Hela, rat epididymis tissue, human tonsil tissue, human kidney tissue, 293T.

【Conjugate】Non-conjugated

【Alternative names】CISY_HUMAN antibody
Citrate synthase antibody
Citrate synthase, mitochondrial antibody
citrate synthetase antibody
Cs antibody
EC 2.3.3 antibody
EC 2.3.3.1 antibody

【Form】Liquid

【Stroage】Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

【Stroage Buffer】1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

【Isotype】IgG

【Purity】Protein A purified.

【Subcellular location】Mitochondrion matrix.

【Background References】1. Redmann M et al. Inhibition of autophagy with bafilomycin and chloroquine decreases mitochondrial quality and bioenergetic function in primary neurons. Redox Biol 11:73-81 (2017).
2. Giroud M et al. Let-7i-5p represses brite adipocyte function in mice and humans. Sci Rep 6:28613 (2016).

【DatasheetURL】https://www.huabio.com/upload/ET1706-40.pdf

【Uniprot ID】O75390(Human) Q9CZU6(Mouse) Q8VHF5(Rat)

【Raised in】Rabbit

【Clone No.】JU80-30