美国BD采血管 366448 蛋白组学P100采血管 BD™ P100 Blood Collection System 366422 366420 366421

• Tube MaterialPlastic
• Tube Size16x100 mm
• Draw Volume8.5  mL
• Closure TypeBD Hemogard™
• Closure ColorClear
• LabelPaper
• Cell SeparatorYes (Mechanical)
• AnticoagulantK₂EDTA
• AdditiveProprietary Protein Stabilizers
• SterilizationRadiation ,  10^-6 SAL ,  ISO11137-1

Description

Human plasma contains a wide variety of proteins that hold potential as biomarkers for proteomics-based diagnostic and prognostic assays. These proteins are subject to proteolytic degradation and modification during and after blood collection.

The BD™ P100 blood collection tubes minimize preanalytical variability in plasma protein analysis. The tubes are spray coated with K₂EDTA anticoagulant and proprietary protease inhibitor cocktail, specifically formulated for stabilization of human plasma proteins at the point of collection. Inclusion of a novel mechanical separator within the 8.5 mL BD™ P100 Tubes provides better separation of cellular components from plasma than gel-based separators.

BD™ P100 is for research use only – it is not for use in diagnostic procedures.

 

PREPARATION AND STORAGE

Store tubes at 4-25 ºC (39-77 ºF) unless otherwise noted on the package label. It is recommended to store the tubes in the original sealed packaging until use. The pouch with desiccant provides a moisture barrier to preserve the potency of the additives.

 

1. Craft D, Yi J, Gelfand CA. Time-dependent and Sample-to-sample Variations in Human Plasma Peptidome are both minimised through use of Protease Inhibitors. Analytical Letters 2009;2:1398-1406.

2. Roy M. Controlled Analysis of Preanalytical Variables in CSF and Blood Sample Collection, Processing and Storage: Implications for Best Practices in Clinical Research. Presented at ISBER, Orlando, FL, 2014.

3. Waters J, Dhere V, Benjamin A, Sekar A, Kumar A, Prahalad S, Okou DT, Kugathasan S. A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation. J Vis Exp. 2013; 75:e4241.

4. Yi J, Craft D, Gelfand CA. Minimising Preanalytical and Analytical Variability in Plasma Peptidomics using MALDI-TOF MS. Methods Mol Biol 2011;728:137-149.

5. Yi J, et al. Inhibition of Intrinsic Proteolytic Activities Moderates Preanalytical Variability and Instability of Human Plasma. J Proteomic Res 2007;6:1768-1781.

6. Yi J, Liu Z, Craft D, O'Mullan P, Ju G, Gelfand CA. Intrinsic Peptidase Activity Causes a Sequential Multi-step Reaction (SMSR) in Digestion of Human Plasma Peptides. J Proteomic Res 2008;7:5112-5118.