Rhizopus oryzae Went et Prinsen Geerligs 拉丁名
ATCC 9363 编号
Strain Designations 菌种别名 NRRL 395 [IMI 40564]
Application 用途
Degrades barley
Degrades cassava
Degrades corn
Degrades rice
Degrades sugarcane bagasse
Produces L(+)-lactic acid
Produces NAD-dependent lactate dehydrogenase
Produces amylase
Produces chitosan
Produces ergostadientriols
Produces fumaric acid
Produces lactic acid lactate
Produces ergostadientriols useful in lowering serum cholesterol levels
Produces L(+)-lactic acid from ground corn
Produces L(+)-lactic acid from barley, cassava, corn, oats and rice
Produces L(+)-lactic acid in calcium alginate gels
Produces L(+)-lactic acid from sugarcane bagasse
Produces amylolytic enzymes
Biosafety Level 安全等级 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Storage Conditions 培养条件
Frozen 冷冻物 : -80°C or colder
Freeze-Dried 冷冻物 : 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Preceptrol no
Comments
Source of lactate dehydrogenase genes used in the construction of expression vectors
Tempeh fermentation
Medium 培养基 ATCC Medium 200: YM agar or YM broth
ATCC Medium 323: Malt agar medium
ATCC Medium 336: Potato dextrose agar (PDA)
Growth Conditions 生长条件
Temperature 培养温度 : 24°C to 26°C
Atmosphere 需氧情况 : Typical aerobic
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence
Isolation 分离基物
Source of lactate dehydrogenase genes used in the construction of expression vectors
Cross References
Nucleotide (GenBank) : AF226154 lactate dehydrogenase (ldhA) gene, complete coding sequence
Nucleotide (GenBank) : AF226155 lactate dehydrogenase (ldhB) gene, complete coding sequence
References 参考文献
Goldberg I, Stieglitz B. Improved rate of fumaric acid production by Tweens and vegetable oils in Rhizopus arrhizus. Biotechnol. Bioeng. 27: 1067-1069, 1985.
Zilliken FW. Ergostadienetriols, compositions containing same, and methods of preparing and using same. US Patent 4,234,577 dated Nov 18 1980
Soccol CR, et al. Potential of solid state fermentation for production of L(+)-lactic acid by Rhizopus oryzae. Appl Microbiol Biotechnol 41: 286-290, 1994.
Yu RC, Hang YD. Purification and characterization of NAD-dependent lactate dehydrogenase from Rhizopus oryzae. Food Chem. 41: 219-225, 1991.
Skory CD. Isolation and expression of lactate dehydrogenase genes from Rhizopus oryzae. Appl. Environ. Microbiol. 66: 2343-2348, 2000. PubMed: 10831409
Skory CD. Fungal lactate dehydrogenase gene and constructs for the expression thereof. US Patent 6,368,189 dated Jul 31 2001
Hang YD. Direct fermentation of corn to L(+)-lactic acid by Rhizopus oryzae. Biotechnol. Lett. 11: 299-300, 1989.
Hang YD, et al. Production of L(+)-lactic acid by Rhizopus oryzae immobilized in calcium alginate gels. Biotechnol. Lett. 11: 119-120, 1989.
Yu RC, Hang YD. Kinetics of direct fermentation of agricultural commodities to L(+)-lactic acid by Rhizopus oryzae. Biotechnol. Lett. 11: 597-600, 1989.
Yu RC, Hang YD. Amylolytic enzyme production by Rhizopus oryzae grown on agricultural commodities. World J. Microbiol. Biotechnol. 6: 15-18, 1990.
Hang YD. Chitosan production from Rhizopus oryzae mycelia. Biotechnol. Lett. 12: 911-912, 1990.
Soccol CR, et al. Production of L-lactic acid by Rhizopus species. World J. Microbiol. Biotechnol. 10: 433-435, 1994.
Pritchard GG. Factors affecting the activity and synthesis of NAD-dependent lactate dehydrogenase in Rhizopus oryzae. J. Gen. Microbiol. 78: 125-137, 1973.
Ward GE, et al. Biochemical studies in the genus Rhizopus. I. The production of dextro-lactic acid. J. Am. Chem. Soc. 58: 1286-1288, 1936.
Gryganskyi AP, et al. Structure, function, and phylogeny of the mating locus in the Rhizopus oryzae complex. PLoS One 5: e15273, 2010. PubMed: 21151560
Skory CD, et al. Analysis of a functional lactate permease in the fungus Rhizopus. Enzyme Microb Technol 46: 43-50, 2010.
Mertens JA, Skory CD. Isolation and characterization of a second glucoamylase gene without a starch binding domain from Rhizopus oryzae. Enzyme Microb Technol 40: 874-880, 2007.
Mertens JA, Skory CD, Ibrahim AS. Plasmids for expression of heterologous proteins in Rhizopus oryzae. Arch Microbiol 186: 41-50, 2006. PubMed: 16804680
Saito K, et al. Genetic diversity in Rhizopus oryzae strains as revealed by the sequence of lactate dehydrogenase genes. Arch Microbiol 182: 30-36, 2004. PubMed: 15278242
Abe A, et al. rDNA ITS sequence of Rhizopus oryzae: its application to classification and identification of lactic acid producers. Biosci Biotechnol Biochem 67: 1725-1731, 2003. PubMed: 12951506
Skory CD. Induction of Rhizopus oryzae pyruvate decarboxylase genes. Curr Microbiol 47: 59-64, 2003. PubMed: 12783195
Skory CD. Homologous recombination and double-strand break repair in the transformation of Rhizopus oryzae. Mol Genet Genomics 268: 397-406, 2002. PubMed: 12436261
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