Product Name:Human Crossover junction endonuclease EME1, EME1 ELISA KIT
Packing:96T
Catalog No.:ELI-47505h
Gene Name:EME1
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Crossover junction endonuclease EME1(EME1
Alternative Name:EME1,Crossover junction endonuclease EME1(EME1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Crossover junction endonuclease EME1, EME1 ELISA KIT allows for the in vitro quantitative determination of Crossover junction endonuclease EME1(EME1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Crossover junction endonuclease EME1, EME1 ELISA KIT has been pre-coated with an Crossover junction endonuclease EME1(EME1 antibody specific to Crossover junction endonuclease EME1(EME1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Crossover junction endonuclease EME1(EME1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Crossover junction endonuclease EME1(EME1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Crossover junction endonuclease EME1(EME1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
