Product Name:Human kappa immunoglobulin light chain ELISA Kit
Packing:96T
Catalog No.:ELA-E0644h
Gene Name:kappa-IgLC
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:kappa immunoglobulin light chain
Alternative Name:kappa-IgLC,kappa immunoglobulin light chain
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human kappa immunoglobulin light chain ELISA Kit allows for the in vitro quantitative determination of kappa immunoglobulin light chain concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human kappa immunoglobulin light chain ELISA Kit has been pre-coated with an kappa immunoglobulin light chain antibody specific to kappa immunoglobulin light chain .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for kappa immunoglobulin light chain and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain kappa immunoglobulin light chain, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of kappa immunoglobulin light chain in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
