Mouse Urokinase plasminogen activator, uPA ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
uPA,ATF; BDPLT5; QPD; URK; u-PA; U-plasminogen activator; plasminogen activator urinary; plasminogen activator urokinase, Urokinase-type plasminogen activator
Search name
Mouse uPA ELISA KIT ,Mouse ATF ELISA KIT ,Mouse BDPLT5 ELISA KIT ,Mouse QPD ELISA KIT ,Mouse URK ELISA KIT ,Mouse u-PA ELISA KIT ,Mouse U-plasminogen activator ELISA KIT ,Mouse plasminogen activator urinary ELISA KIT ,Mouse plasminogen activator urokinase ELISA KIT ,Mouse Urokinase-type plasminogen activator ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse urokinase-type plasminogen activator,uPA concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to uPA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for uPA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain uPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of uPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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