Mouse Soluble Low-density lipoprotein receptor-related protein 11, sLRP-11 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Soluble Low-density lipoprotein receptor-related protein 11(sLRP-11),;
Search name
Mouse Soluble Low-density lipoprotein receptor-related protein 11 ELISA Kit, Mouse sLRP-11 ELISA Kit, Mouse sLRP11 ELISA Kit, Mouse soluble LRP11 ELISA Kit, Mouse soluble MANSC3 ELISA Kit, Mouse soluble bA350J20.3 ELISA Kit, Mouse solublelow density lipoprotein receptor-related protein 11ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse soluble low-density lipoprotein receptor-related protein 11,sLRP-11 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to sLRP-11. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for sLRP-11 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.Only those wells that contain sLRP-11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of sLRP-11 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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