Mouse Ribose- 5- phosphate isomerase, RPIA ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Class 5.3.1.6,Ribose-5-phosphate isomerase),Phosphoriboisomerase,RPIA,RPI,phosphoriboisomerase,ribose 5-phosphate epimerase,ribose-5-phosphate isomerase,ribose 5-phosphate isomerase A
Search name
Mouse Class 5.3.1.6 ELISA KIT ,Mouse Ribose-5-phosphate isomerase) ELISA KIT ,Mouse Phosphoriboisomerase ELISA KIT ,Mouse RPIA ELISA KIT ,Mouse RPI ELISA KIT ,Mouse phosphoriboisomerase ELISA KIT ,Mouse ribose 5-phosphate epimerase ELISA KIT ,Mouse ribose-5-phosphate isomerase ELISA KIT ,Mouse ribose 5-phosphate isomerase A ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse ribose-5-phosphate isomerase,RPIA concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RPIA, During the reaction, RPIA in the sample or standard competes with a fixed amount of biotin-labeled RPIA for sites on a pre-coated Monoclonal antibody specific to RPIA. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of RPIA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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