Mouse Adenosine deaminase- like protein, ADAL ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Adenosine deaminase-like protein, ADAL,adenosine deaminase-like
Search name
Mouse ADAL ELISA Kit, Mouse Adenosine deaminase-like protein ELISA Kit, Mouse adenosine deaminase-like ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Adenosine deaminase-like protein concentrations in
serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Adenosine deaminase-like protein, During the reaction, Adenosine deaminase-like protein in the
sample or standard competes with a fixed amount of biotin-labeled Adenosine deaminase-like protein for sites on a pre-coated
Monoclonal antibody specific to Adenosine deaminase-like protein. Excess conjugate and unbound sample or standard are washed from
the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB
substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the
color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Adenosine deaminase-like
protein in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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